• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Molecular and Cellular Biology >Transcriptional modulation of transin gene expression by epidermal growth factor and transforming growth factor beta.
【24h】

Transcriptional modulation of transin gene expression by epidermal growth factor and transforming growth factor beta.

机译：表皮生长因子和转化生长因子β的转录调节transin基因表达。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Transin is a transformation-associated gene which is expressed constitutively in rat fibroblasts transformed by a variety of oncogenes and in malignant mouse skin carcinomas but not benign papillomas or normal skin. It has been demonstrated that, in nontransformed Rat-1 cells, transin RNA expression is modulated positively by epidermal growth factor (EGF) and negatively by transforming growth factor beta (TGF-beta); other peptide growth factors were found to have no effect on transin expression. Results presented here indicate that both protein synthesis and continuous occupancy of the EGF receptor by EGF were required for sustained induction of transin RNA. Treatment with TGF-beta inhibited the ability of EGF to induce transin, whether assayed at the transcriptional level by nuclear run-on analysis or at the level of transin RNA accumulation by Northern (RNA) blot analysis of cellular RNA. TGF-beta both blocked initial induction of transin transcription by EGF and halted established production of transin transcripts during prolonged treatment. These results suggest that TGF-beta acts at the transcriptional level to antagonize EGF-mediated induction of transin gene expression.
机译：转蛋白是与转化相关的基因，其在由多种癌基因转化的大鼠成纤维细胞中组成性表达，并在恶性小鼠皮肤癌中表达，但在良性乳头状瘤或正常皮肤中不表达。已经证明，在未转化的Rat-1细胞中，转基因RNA的表达受表皮生长因子（EGF）的正调控，而受转化生长因子β（TGF-β）的负调控。发现其他肽生长因子对转基因表达没有影响。此处呈现的结果表明，蛋白质合成和EGF对EGF受体的持续占据都是持续诱导转导RNA所必需的。无论是在核转录分析中在转录水平上还是在细胞RNA的Northern（RNA）印迹分析中以转录水平上的RNA积累水平测定，用TGF-β处理均能抑制EGF诱导转座蛋白的能力。 TGF-β既阻止了EGF对转导蛋白转录的初始诱导，又阻止了长期治疗过程中已经建立的转导蛋白转录产物的产生。这些结果表明，TGF-β在转录水平上起拮抗EGF介导的转导基因表达的诱导作用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号