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首页> 外文期刊>Molecular and Cellular Biology >Nonsense codons within the Rous sarcoma virus gag gene decrease the stability of unspliced viral RNA.
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Nonsense codons within the Rous sarcoma virus gag gene decrease the stability of unspliced viral RNA.

机译：Rous肉瘤病毒gag基因中的无意义密码子降低了未剪接病毒RNA的稳定性。

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The intracellular accumulation of the unspliced RNA of Rous sarcoma virus was decreased when translation was prematurely terminated by the introduction of nonsense codons within its 5' proximal gene, the gag gene. In contrast, the levels of spliced viral RNAs were not affected in our transient expression assays in chicken cells. Experiments using the transcription inhibitor dactinomycin showed that mutant unspliced RNAs were degraded more rapidly than wild-type RNA. Furthermore, mutant RNAs could be partially stabilized by coexpression of wild-type gag proteins in trans; however, intact gag proteins were not required to maintain the stability of RNAs which did not contain premature termination codons. Thus, termination codons seemed to destabilize the RNA not because of their effect on gag protein function but instead because they disrupted the process of translating the gag region of the RNA. Analysis of double-mutant constructs containing both deletions and termination codons within the gag gene also suggested that the stability of the unspliced RNA was affected by a cis-acting interaction between the RNA and ribosomes.
机译：当通过在其5'近端基因gag基因中引入无义密码子而过早终止翻译时，劳斯肉瘤病毒未剪接RNA的细胞内积累减少。相反，在我们的鸡细胞瞬时表达分析中，剪接病毒RNA的水平没有受到影响。使用转录抑制剂放线菌素的实验表明，突变的未剪接RNA比野生型RNA降解得更快。此外，突变型RNA可以通过野生型gag蛋白反式共表达而部分稳定。但是，不需要完整的gag蛋白来维持不包含过早终止密码子的RNA的稳定性。因此，终止密码子似乎使RNA不稳定，不是因为它们对gag蛋白功能的影响，而是因为它们破坏了翻译RNA的gag区的过程。分析包含在gag基因中的缺失和终止密码子的双突变体构建体，还表明未剪接的RNA的稳定性受RNA与核糖体之间顺式作用相互作用的影响。

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