• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Virology >Efficient transformation by Prague A Rous sarcoma virus plasmid DNA requires the presence of cis-acting regions within the gag gene.
【24h】

Efficient transformation by Prague A Rous sarcoma virus plasmid DNA requires the presence of cis-acting regions within the gag gene.

机译：布拉格的高效转化是肉瘤病毒质粒DNA需要在GAG基因内存在顺式作用区。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

A region in addition to and outside the long terminal repeats (LTRs) in the gag gene of the Prague A strain of Rous sarcoma virus was found to be essential in cis for efficient cell transformation by cloned viral DNA. Transformation in chicken embryo fibroblasts, which requires infectious virus production and reinfection, was facilitated in cis by sequences between nucleotides 630 and 1659. Efficient transformation of NIH 3T3 cells in which secondary spread of virus is not necessary (as it is in chicken embryo fibroblasts) required sequences between nucleotides 630 and 1149. A src cDNA clone which also lacks this region demonstrated low transformation efficiency, indicating that the role of the cis element cannot be attributed to interference with RNA splicing. The gag gene segment required in cis for transformation, between nucleotides 630 and 1149, could substitute for the simian virus 40 enhancer in either orientation, and cells transfected with Rous sarcoma virus LTR-driven plasmids containing the gag cis element had a two- to threefold increase in steady-state viral RNA levels compared with plasmids lacking this region. Thus, additional cis-acting regulatory elements located outside the viral LTRs may modulate viral gene expression and contribute to the efficiency of cell transformation.
机译：除了布拉格的GAG基因中的长终端重复（LTR）之外的一个区域，该区域是由克隆病毒DNA的有效细胞转化的CIS中的基因中的一种区域。通过核苷酸630和1659之间的序列促进了鸡胚胚胎成纤维细胞的转化，该胚胎成纤维细胞在CIS中促进了CIS中的序列。没有必要的NIH 3T3细胞的有效转化，其中病毒的二次扩散（如鸡胚成纤维细胞）核苷酸630和1149之间的所需序列。SRC cDNA克隆，也缺乏该区域的低变化效率，表明顺式元素的作用不能归因于干扰RNA剪接。在核苷酸630和1149之间转化的CIS中所需的GAG基因链段可以替代含有含有GAG CIS元素的ROS Sarcoma病毒LTR驱动的细胞的猿猴病毒40增强剂的细胞具有两个至三倍与缺乏该区域的质粒相比，稳态病毒RNA水平增加。因此，位于病毒LTR外部的附加顺式作用调节元件可以调节病毒基因表达并有助于细胞转化的效率。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号