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首页> 外文期刊>Molecular and Cellular Biology >The human CCG1 gene, essential for progression of the G1 phase, encodes a 210-kilodalton nuclear DNA-binding protein.
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The human CCG1 gene, essential for progression of the G1 phase, encodes a 210-kilodalton nuclear DNA-binding protein.

机译：人类CCG1基因对G1期的发展至关重要，它编码210千达尔顿核DNA结合蛋白。

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摘要

The human CCG1 gene complements tsBN462, a temperature-sensitive G1 mutant of the BHK21 cell line. The previously cloned cDNA turned out to be a truncated form of the actual CCG1 cDNA. The newly cloned CCG1 cDNA was 6.0 kb and encoded a protein with a molecular mass of 210 kDa. Using an antibody to a predicted peptide from the CCG1 protein, a protein with a molecular mass of over 200 kDa was identified in human, monkey, and hamster cell lines. In the newly defined C-terminal region, an acidic domain was found. It contained four consensus target sequences for casein kinase II and was phosphorylated by this enzyme in vitro. However, this C-terminal region was not required to complement tsBN462 mutation since the region encoding the C-terminal part was frequently missing in complemented clones derived by DNA-mediated gene transfer. CCG1 contains a sequence similar to the putative DNA-binding domain of HMG1 in addition to the previously detected amino acid sequences common in nuclear proteins, such as a proline cluster and a nuclear translocation signal. Consistent with these predictions, CCG1 was present in nuclei, possessed DNA-binding activity, and was eluted with similar concentrations of salt, 0.3 to 0.4 M NaCl either from isolated nuclei or from a DNA-cellulose column.
机译：人类CCG1基因与tsBN462互补，tsBN462是BHK21细胞系的温度敏感型G1突变体。先前克隆的cDNA原来是实际CCG1 cDNA的截短形式。新克隆的CCG1 cDNA为6.0 kb，编码分子量为210 kDa的蛋白质。使用来自CCG1蛋白的预测肽的抗体，在人，猴和仓鼠细胞系中鉴定出分子量超过200 kDa的蛋白。在新定义的C端区域，发现一个酸性域。它包含酪蛋白激酶II的四个共有靶序列，并且在体外被该酶磷酸化。但是，不需要该C末端区域来补充tsBN462突变，因为在通过DNA介导的基因转移衍生的互补克隆中，经常缺少编码C末端部分的区域。 CCG1除先前检测到的核蛋白中常见的氨基酸序列（例如脯氨酸簇和核易位信号）外，还包含与HMG1推定的DNA结合域相似的序列。与这些预测一致，CCG1存在于细胞核中，具有DNA结合活性，并用相似浓度的盐，0.3-0.4 M NaCl从分离的细胞核或DNA纤维素柱中洗脱。

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