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首页> 外文期刊>Molecular and Cellular Biology >Molecular analyses of two poly(A) site-processing factors that determine the recognition and efficiency of cleavage of the pre-mRNA.
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Molecular analyses of two poly(A) site-processing factors that determine the recognition and efficiency of cleavage of the pre-mRNA.

机译：对两个poly（A）加工位点决定因素的分子分析，这些因素决定了前mRNA的识别和切割效率。

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摘要

Poly(A) site processing of a pre-mRNA requires the participation of multiple nuclear factors. Two of these factors recognize specific sequences in the pre-mRNA and form a stable processing complex. Since these initial interactions are likely critical for the recognition of the poly(A) site and the efficiency of poly(A) site use, we have characterized these factors and the nature of their interaction with the pre-mRNA. The AAUAAA specificity factor PF2 is a large, multicomponent complex composed of at least five distinct polypeptides ranging in molecular size from 170 to 42 kDa. The 170-kDa polypeptide appears to mediate interaction with the pre-mRNA. Factor CF1, which provides specificity for the downstream G + U-rich element and stabilizes the PF2 interaction on the RNA, is also a multicomponent complex but is less complex than PF2. CF1 is composed of three polypeptides of molecular sizes 76, 64, and 48 kDa. UV cross-linking assays demonstrate that the 64-kDa polypeptide makes direct contact with the RNA, dependent on the G + U-rich downstream sequence element. Moreover, it is clear that these RNA-protein interactions are influenced by the apparent cooperative interaction involving PF2 and CF1, interactions that contribute to the efficiency of poly(A) site processing.
机译：前mRNA的Poly（A）加工需要多个核因子的参与。这些因素中的两个识别前mRNA中的特定序列并形成稳定的加工复合物。由于这些最初的相互作用对于识别poly（A）位点和使用poly（A）位点的效率可能至关重要，因此我们已经表征了这些因素及其与pre-mRNA相互作用的性质。 AAUAAA特异性因子PF2是一种大的多组分复合物，由至少五个不同的多肽组成，其分子大小在170至42kDa之间。 170-kDa多肽似乎介导与前mRNA的相互作用。 CF1因子对下游富含G + U的元素具有特异性，并稳定PF2在RNA上的相互作用，它也是一种多组分复合物，但不如PF2复杂。 CF1由分子大小为76、64和48 kDa的三个多肽组成。紫外线交联测定表明，64-kDa多肽直接与RNA接触，这取决于富含G + U的下游序列元件。此外，很明显，这些RNA-蛋白质相互作用受到涉及PF2和CF1的明显协同相互作用的影响，这些相互作用有助于poly（A）位点加工的效率。

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