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首页> 外文期刊>Molecular and Cellular Biology >The PHO84 gene of Saccharomyces cerevisiae encodes an inorganic phosphate transporter.
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The PHO84 gene of Saccharomyces cerevisiae encodes an inorganic phosphate transporter.

机译：酿酒酵母的PHO84基因编码无机磷酸盐转运蛋白。

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摘要

The PHO84 gene specifies Pi-transport in Saccharomyces cerevisiae. A DNA fragment bearing the PHO84 gene was cloned by its ability to complement constitutive synthesis of repressible acid phosphatase of pho84 mutant cells. Its nucleotide sequence predicted a protein of 596 amino acids with a sequence homologous to that of a superfamily of sugar transporters. Hydropathy analysis suggested that the secondary structure of the PHO84 protein consists of two blocks of six transmembrane domains separated by 74 amino acid residues. The cloned PH084 DNA restored the Pi transport activity of pho84 mutant cells. The PHO84 transcription was regulated by Pi like those of the PHO5, PHO8, and PHO81 genes. A PHO84-lacZ fusion gene produced beta-galactosidase activity under the regulation of Pi, and the activity was suggested to be bound to a membrane fraction. Gene disruption of PHO84 was not lethal. By comparison of nucleotide sequences and by tetrad analysis with GAL80 as a standard, the PHO84 locus was mapped at a site beside the TUB3 locus on the left arm of chromosome XIII.
机译：PHO84基因指定啤酒酵母中的Pi转运。通过其互补pho84突变细胞的可抑制性酸性磷酸酶组成型合成的能力，克隆了带有PHO84基因的DNA片段。它的核苷酸序列预测了一个596个氨基酸的蛋白质，其序列与糖转运蛋白超家族的同源。亲水性分析表明，PHO84蛋白的二级结构由被74个氨基酸残基分隔的六个跨膜结构域的两个嵌段组成。克隆的PH084 DNA恢复了pho84突变细胞的Pi转运活性。 Pi像PHO5，PHO8和PHO81基因一样，通过Ph调节PHO84的转录。一个PHO84-lacZ融合基因在Pi的调节下产生了β-半乳糖苷酶的活性，并且该活性被认为与膜部分结合。 PHO84的基因破坏并不致命。通过比较核苷酸序列和以GAL80为标准品进行四联体分析，将PHO84基因座定位在XIII染色体左臂上TUB3基因座旁边的位点。

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