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首页> 外文期刊>Molecular and Cellular Biology >Differential binding of zinc fingers from Xenopus TFIIIA and p43 to 5S RNA and the 5S RNA gene.
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Differential binding of zinc fingers from Xenopus TFIIIA and p43 to 5S RNA and the 5S RNA gene.

机译：非洲爪蟾TFIIIA和p43的锌指与5S RNA和5S RNA基因的差异结合。

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摘要

Zinc fingers are usually associated with proteins that interact with DNA. Yet in two oocyte-specific Xenopus proteins, TFIIA and p43, zinc fingers are used to bind 5S RNA. One of these, TFIIIA, also binds the 5S RNA gene. Both proteins have nine zinc fingers that are nearly identical with respect to size and spacing. We have determined the relative affinities of groups of zinc fingers from TFIIIA for both 5S RNA and the 5S RNA gene. We have also determined the relative affinities of groups of zinc fingers from p43 for 5S RNA. The primary protein regions for RNA and DNA interaction in TFIIIA are located at opposite ends of the molecule. All zinc fingers from TFIIIA participate in binding 5S RNA, but zinc fingers from the C terminus have the highest affinity. N-terminal zinc fingers are essential for binding the 5S RNA gene. In contrast, zinc fingers at the amino terminus of p43 are essential for binding 5S RNA.
机译：锌指通常与与DNA相互作用的蛋白质有关。然而，在两种卵母细胞特异性非洲爪蟾蛋白TFIIA和p43中，锌指用于结合5S RNA。其中之一，TFIIIA，也与5S RNA基因结合。两种蛋白质都有九个锌指，它们的大小和间距几乎相同。我们已经确定了来自TFIIIA的锌指对5S RNA和5S RNA基因的相对亲和力。我们还确定了来自p43的锌指对5S RNA的相对亲和力。 TFIIIA中用于RNA和DNA相互作用的主要蛋白质区域位于分子的相对末端。来自TFIIIA的所有锌指均参与结合5S RNA，但来自C末端的锌指具有最高的亲和力。 N末端锌指对于结合5S RNA基因至关重要。相反，在p43氨基末端的锌指对于结合5S RNA是必不可少的。

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