Conserved DNA binding and self-association domains of the Drosophila zeste protein.

J D Chen; C S Chan; V Pirrotta

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Conserved DNA binding and self-association domains of the Drosophila zeste protein.

机译：果蝇zeste蛋白的保守的DNA结合和自我缔合域。

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The zeste gene product is involved in two types of genetic effects dependent on chromosome pairing: transvection and the zeste-white interaction. Comparison of the predicted amino acid sequence with that of the Drosophila virilis gene shows that several blocks of amino acid sequence have been very highly conserved. One of these regions corresponds to the DNA binding domain. Site-directed mutations in this region indicate that a sequence resembling that of the homeodomain DNA recognition helix is essential for DNA binding activity. The integrity of an amphipathic helical region is also essential for binding activity and is likely to be responsible for dimerization of the DNA binding domain. Another very strongly conserved domain of zeste is the C-terminal region, predicted to form a long helical structure with two sets of heptad repeats that constitute two long hydrophobic ridges at opposite ends and on opposite faces of the helix. We show that this domain is responsible for the extensive aggregation properties of zeste that are required for its role in transvection phenomena. A model is proposed according to which the hydrophobic ridges induce the formation of open-ended coiled-coil structures holding together many hundreds of zeste molecules and possibly anchoring these complexes to other nuclear structures.

机译：zeste基因产物参与两种取决于染色体配对的遗传效应：平移和zeste-white相互作用。将预测的氨基酸序列与果蝇基因的氨基酸序列进行比较表明，氨基酸序列的几个区块已经非常保守。这些区域之一对应于DNA结合结构域。该区域中的定点突变表明，类似于同源域DNA识别螺旋的序列对于DNA结合活性至关重要。两亲性螺旋区的完整性对于结合活性也是必不可少的，并且可能负责DNA结合域的二聚化。 zeste的另一个非常保守的结构域是C末端区域，预计将形成一个长螺旋结构，该结构具有两组七聚体重复序列，这些重复序列在螺旋的相对两端和相对面上构成两个长疏水性脊。我们显示该域负责zeste的广泛聚集特性，这些特性是其在对流现象中的作用所必需的。提出了一种模型，根据该模型，疏水性脊诱导形成将数百个zeste分子聚集在一起并可能将这些复合物锚定到其他核结构的开放式卷曲螺旋结构。

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《Molecular and Cellular Biology》 |1992年第2期|共11页
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J D Chen; C S Chan; V Pirrotta;
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中图分类细胞生物学;
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1. The DNA-binding and enhancer-blocking domains of the Drosophila suppressor of Hairy-wing protein. [J] . J Kim, B Shen, C Rosen, Molecular and Cellular Biology . 1996,第7期

机译：果蝇翅果蝇抑制剂的DNA结合和增强子阻断域。
2. The Drosophila tissue-specific factor Grainyhead contains novel DNA-binding and dimerization domains which are conserved in the human protein CP2. [J] . A E Uv, C R Thompson, S J Bray Molecular and Cellular Biology . 1994,第6期

机译：果蝇组织特异性因子粒头含有新的DNA结合和二聚化域，在人类蛋白CP2中是保守的。
3. Synergistic activation of transcription is mediated by the N-terminal domain of Drosophila fushi tarazu homeoprotein and can occur without DNA binding by the protein. [J] . J Ananthan, R Baler, D Morrissey, Molecular and Cellular Biology . 1993,第3期

机译：转录的协同激活是由果蝇Fushi tarazu同源蛋白的N-末端结构域介导的，并且可以在没有DNA与蛋白结合的情况下发生。
4. Evolutionary conservation of DNA-contact residues in DNA-binding domains [C] . Chang, Yao-Lin, Tsai, Computer and Computational Sciences (IMSCCS), 2007 Second International Multisymposium on . 2007

机译：DNA结合域中DNA接触残基的进化保守性
5. I. Cloning and characterization of human DNA polymerase theta. II. Protein interactions of human damaged DNA binding protein. [D] . Abbas, Alexander Rodney. 2000

机译：I.人DNA聚合酶θ的克隆和表征。二。人类受损的DNA结合蛋白的蛋白质相互作用。
6. Conserved DNA binding and self-association domains of the Drosophila zeste protein. [O] . J D Chen, C S Chan, V Pirrotta 1992

机译：果蝇zeste蛋白的保守的DNA结合和自缔合域。
7. Conserved DNA binding and self-association domains of the Drosophila zeste protein. [O] . Chen, J D, Chan, C S, Pirrotta, V 1992

机译：果蝇zeste蛋白的保守的DNA结合和自缔合域。

Conserved DNA binding and self-association domains of the Drosophila zeste protein.

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