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首页> 外文期刊>Molecular and Cellular Biology >DNA repair synthesis during base excision repair in vitro is catalyzed by DNA polymerase epsilon and is influenced by DNA polymerases alpha and delta in Saccharomyces cerevisiae.
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DNA repair synthesis during base excision repair in vitro is catalyzed by DNA polymerase epsilon and is influenced by DNA polymerases alpha and delta in Saccharomyces cerevisiae.

机译：DNA聚合酶epsilon催化体外碱基切除修复过程中的DNA修复合成，并受到酿酒酵母中DNA聚合酶α和δ的影响。

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摘要

Base excision repair is an important mechanism for correcting DNA damage produced by many physical and chemical agents. We have examined the effects of the REV3 gene and the DNA polymerase genes POL1, POL2, and POL3 of Saccharomyces cerevisiae on DNA repair synthesis is nuclear extracts. Deletional inactivation of REV3 did not affect repair synthesis in the base excision repair pathway. Repair synthesis in nuclear extracts of pol1, pol2, and pol3 temperature-sensitive mutants was normal at permissive temperatures. However, repair synthesis in pol2 nuclear extracts was defective at the restrictive temperature of 37 degrees C and could be complemented by the addition of purified yeast DNA polymerase epsilon. Repair synthesis in pol1 nuclear extracts was proficient at the restrictive temperature unless DNA polymerase alpha was inactivated prior to the initiation of DNA repair. Thermal inactivation of DNA polymerase delta in pol3 nuclear extracts enhanced DNA repair synthesis approximately 2-fold, an effect which could be specifically reversed by the addition of purified yeast DNA polymerase delta to the extract. These results demonstrate that DNA repair synthesis in the yeast base excision repair pathway is catalyzed by DNA polymerase epsilon but is apparently modulated by the presence of DNA polymerases alpha and delta.
机译：碱基切除修复是纠正许多物理和化学试剂产生的DNA损伤的重要机制。我们已经检查了酿酒酵母的REV3基因和DNA聚合酶基因POL1，POL2和POL3对DNA修复合成的影响是核提取物。 REV3的理想失活不影响碱基切除修复途径中的修复合成。 pol1，pol2和pol3温度敏感突变体的核提取物中的修复合成在允许温度下是正常的。但是，pol2核提取物中的修复合成在37摄氏度的限制性温度下存在缺陷，并且可以通过添加纯化的酵母DNA聚合酶epsilon进行补充。除非在启动DNA修复之前将DNA聚合酶α灭活，否则在限制温度下pol1核提取物中的修复合成是熟练的。 pol3核提取物中DNA聚合酶δ的热失活使DNA修复合成提高了约2倍，这一作用可以通过在提取物中添加纯化的酵母DNA聚合酶δ来特异地逆转。这些结果表明，在酵母碱基切除修复途径中的DNA修复合成是由DNA聚合酶ε催化的，但是显然受到DNA聚合酶α和δ的存在的调节。

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