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首页> 外文期刊>Molecular and Cellular Biology >A U3 small nuclear ribonucleoprotein-requiring processing event in the 5' external transcribed spacer of Xenopus precursor rRNA.
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A U3 small nuclear ribonucleoprotein-requiring processing event in the 5' external transcribed spacer of Xenopus precursor rRNA.

机译：非洲爪蟾前体rRNA的5'外部转录间隔区中需要U3小核糖核蛋白的加工事件。

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摘要

A processing site has been identified within the 5' external transcribed spacer (ETS) of Xenopus laevis and X. borealis pre-RNAs, and this in vivo processing can be reproduced in vitro. It involves a stable and specific association of the pre-rRNA with factors in the cell extract, including at least four RNA-contacting polypeptides, yielding a distinct complex that sediments at 20S. Processing also requires the U3 small nuclear RNA. This processing, at residue +105 of the 713-nucleotide X. laevis 5' ETS, is highly reminiscent of the initial processing cleavage of mouse pre-rRNA within its 3.5-kb 5' ETS, previously thought to be mammal specific. The frog and mouse processing signals share a short essential sequence motif, and mouse factors can faithfully process the frog pre-rRNA. This conservation suggests that this 5' ETS processing site serves an evolutionarily selective function.
机译：已经在非洲爪蟾和北极X.boalis前RNA的5'外部转录间隔区（ETS）内鉴定了加工位点，并且该体内加工可以在体外复制。它涉及pre-rRNA与细胞提取物中各种因子的稳定和特异性结合，包括至少四个与RNA接触的多肽，产生独特的复合物，该复合物在20S时沉淀。加工还需要U3小核RNA。这种在713个核苷酸的X.laevis 5'ETS残基+105处的加工非常让人联想到小鼠pre-rRNA在其3.5kb 5'ETS中的最初加工裂解，以前认为这是哺乳动物特异性的。青蛙和小鼠的加工信号共有一个短的必需序列基序，并且小鼠因子可以忠实地加工青蛙的前rRNA。这种保守性表明该5'ETS加工位点具有进化选择功能。

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