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首页> 外文期刊>Molecular and Cellular Biology >Control of transcription arrest in intron 1 of the murine adenosine deaminase gene.
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Control of transcription arrest in intron 1 of the murine adenosine deaminase gene.

机译：鼠腺苷脱氨酶基因的内含子1中转录停滞的控制。

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Transcription arrest plays a key role in the regulation of the murine adenosine deaminase (ADA) gene, as well as a number of other cellular and viral genes. We have previously characterized the ADA intron 1 arrest site, located 145 nucleotides downstream of the transcription start site, with respect to sequence and elongation factor requirements. Here, we show that the optimal conditions for both intron 1 arrest and overall ADA transcription involve the addition of high concentrations of KCl soon after initiation. As we have further delineated the sequence requirements for intron 1 arrest, we have found that sequences downstream of the arrest site are unnecessary for arrest. Also, a 24-bp fragment containing sequences upstream of the arrest site is sufficient to generate arrest downstream of the adenovirus major late promoter only in the native orientation. Surprisingly, we found that deletion of sequences encompassing the ADA transcription start site substantially reduced intron 1 arrest, with no effect on overall levels of transcription. At the same time, deletion of sequences upstream of the TATA box had no significant effect on either process. We believe the start site mutations have disrupted either the assembly or the composition of the transcription complex such that intron 1 site read-through is now favored. This finding, coupled with the increase in overall transcription after high-concentration KCl treatment, allows us to further refine our model of ADA gene regulation.
机译：转录抑制在鼠腺苷脱氨酶（ADA）基因以及许多其他细胞和病毒基因的调控中起着关键作用。相对于序列和延伸因子的要求，我们先前已对ADA内含子1停滞位点进行了表征，该位点位于转录起始位点下游145个核苷酸处。在这里，我们表明内含子1逮捕和整个ADA转录的最佳条件涉及启动后不久添加高浓度的KCl。随着我们进一步描述内含子1逮捕的序列要求，我们发现逮捕位点下游的序列对于逮捕是不必要的。而且，包含在停滞位点上游的序列的24bp片段足以仅在天然方向上在腺病毒主要晚期启动子的下游产生停滞。出人意料的是，我们发现包含ADA转录起始位点的序列的缺失大大减少了内含子1的捕获，而对总体转录水平没有影响。同时，TATA框上游序列的删除对这两个过程均无显着影响。我们认为起始位点突变破坏了转录复合物的组装或组成，因此现在更倾向于内含子1位点通读。这一发现，再加上高浓度氯化钾处理后总体转录的增加，使我们能够进一步完善我们的ADA基因调控模型。

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