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首页> 外文期刊>Molecular and Cellular Biology >Complementation of a capsule-deficient mutation of Cryptococcus neoformans restores its virulence.
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Complementation of a capsule-deficient mutation of Cryptococcus neoformans restores its virulence.

机译：补充新隐球菌的荚膜缺陷型突变可恢复其毒力。

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Capsule formation plays a significant role in the pathogenicity of Cryptococcus neoformans. To study the molecular basis of capsule synthesis, the capsule-deficient phenotype of a mutant strain was complemented by transformation. A plasmid rescued from the resulting Cap+ transformant complemented a cap59 mutation which was mapped previously by classical recombination analysis. Gene deletion by homologous integration resulted in an acapsular phenotype, indicating that we have identified the CAP59 gene. The CAP59 gene was assigned to chromosome I by Southern blot analysis of contour-clamped homogeneous electric field gel electrophoresis-resolved chromosomes of C. neoformans var. neoformans. Sequence comparison of genomic and cDNA clones indicated the presence of six introns. CAP59 encoded a 1.9-kb transcript and a deduced protein of 458 amino acids. Analysis of the nucleotide sequence revealed little similarity to existing sequences in the data bank. When the capsule-deficient phenotype was complemented, the originally avirulent C. neoformans strain became virulent for mice. In addition, the acapsular strain created by gene deletion of CAP59 lost its virulence. This work demonstrates the molecular basis for capsule-related virulence and that the CAP59 gene is required for capsule formation.
机译：胶囊的形成在新隐球菌的致病性中起重要作用。为了研究胶囊合成的分子基础，突变菌株的胶囊缺陷表型通过转化来补充。从产生的Cap +转化子中拯救的质粒与cap59突变互补，该突变先前已通过经典重组分析进行了定位。通过同源整合的基因删除导致荚膜表型，表明我们已经确定了CAP59基因。 CAP59基因通过等高线钳均质电场凝胶电泳分辨的新隐梭菌染色体的Southern印迹分析被分配给I号染色体。新甲虫。基因组和cDNA克隆的序列比较表明存在六个内含子。 CAP59编码一个1.9-kb的转录本和一个458个氨基酸的推导蛋白。核苷酸序列的分析显示与数据库中现有序列几乎没有相似性。当缺乏胶囊的表型得到补充时，最初无毒的新孢梭菌菌株对小鼠具有毒性。另外，由CAP59的基因缺失产生的荚膜菌株失去了毒力。这项工作证明了胶囊相关毒力的分子基础，并且CAP59基因是胶囊形成所必需的。

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