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首页> 外文期刊>Molecular and Cellular Biology >The bulk chromatin structure of a murine transgene does not vary with its transcriptional or DNA methylation status.
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The bulk chromatin structure of a murine transgene does not vary with its transcriptional or DNA methylation status.

机译：鼠转基因的整体染色质结构不随其转录或DNA甲基化状态而变化。

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摘要

The DNA methylation status of HRD, a murine transgene, can be controlled by the genetic background upon which it is carried. We found the transgene to be transcribed in competent tissues only when undermethylated. Chromatin structure over the transgene was assayed by nuclear accessibility with DNase I, MspI, and PstI. While the transgene was up to fivefold more resistant to MspI when methylated than when not methylated, we observed no such difference with DNase I or PstI. We suggest that methyl-CpG-binding proteins are responsible for the difference observed with MspI, but that the chromatin structures are otherwise similarly compacted. Methylation could, therefore, play a regulatory role in gene expression beyond that which can be accomplished by bulk chromatin structure alone.
机译：HRD（一种鼠类转基因）的DNA甲基化状态可以通过携带它的遗传背景来控制。我们发现仅当甲基化不足时，转基因才能在感受态组织中转录。通过DNase I，MspI和PstI的核可及性分析了转基因上的染色质结构。虽然转基因甲基化后对MspI的耐受性比未甲基化时高多达五倍，但我们发现DNase I或PstI没有这种差异。我们建议，甲基-CpG结合蛋白是造成MspI差异的原因，但染色质结构在其他方面类似地被压缩。因此，甲基化可以在基因表达中起调节作用，而不仅仅是单独的整体染色质结构可以完成。

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