Yeast mitochondrial RNase P RNA synthesis is altered in an RNase P protein subunit mutant: insights into the biogenesis of a mitochondrial RNA-processing enzyme.

V Stribinskis; G J Gao; P Sulo; Y L Dang; N C Martin

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Yeast mitochondrial RNase P RNA synthesis is altered in an RNase P protein subunit mutant: insights into the biogenesis of a mitochondrial RNA-processing enzyme.

机译：酵母线粒体RNase P RNA的合成在RNase P蛋白亚基突变体中发生了改变：洞察线粒体RNA加工酶的生物发生。

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Rpm2p is a protein subunit of Saccharomyces cerevisiae yeast mitochondrial RNase P, an enzyme which removes 5' leader sequences from mitochondrial tRNA precursors. Precursor tRNAs accumulate in strains carrying a disrupted allele of RPM2. The resulting defect in mitochondrial protein synthesis causes petite mutants to form. We report here that alteration in the biogenesis of Rpm1r, the RNase P RNA subunit, is another consequence of disrupting RPM2. High-molecular-weight transcripts accumulate, and no mature Rpm1r is produced. Transcript mapping reveals that the smallest RNA accumulated is extended on both the 5' and 3' ends relative to mature Rpm1r. This intermediate and other longer transcripts which accumulate are also found as low-abundance RNAs in wild-type cells, allowing identification of processing events necessary for conversion of the primary transcript to final products. Our data demonstrate directly that Rpm1r is transcribed with its substrates, tRNA met f and tRNAPro, from a promoter located upstream of the tRNA met f gene and suggest that a portion also originates from a second promoter, located between the tRNA met f gene and RPM1. We tested the possibility that precursors accumulate because the RNase P deficiency prevents the removal of the downstream tRNAPro. Large RPM1 transcripts still accumulate in strains missing this tRNA. Thus, an inability to process cotranscribed tRNAs does not explain the precursor accumulation phenotype. Furthermore, strains with mutant RPM1 genes also accumulate precursor Rpm1r, suggesting that mutations in either gene can lead to similar biogenesis defects. Several models to explain precursor accumulation are presented.

机译：Rpm2p是酿酒酵母酵母线粒体RNase P的蛋白质亚基，该酶可从线粒体tRNA前体中删除5'前导序列。前体tRNA在携带RPM2等位基因破坏的菌株中积累。线粒体蛋白质合成中产生的缺陷导致小突变体形成。我们在这里报告，Rpm1r，RNase P RNA亚基的生物发生中的改变是破坏RPM2的另一个结果。高分子量的成绩单积累，并且没有成熟的Rpm1r产生。转录本作图揭示了相对于成熟的Rpm1r，积累的最小RNA在5'和3'末端均延伸。在野生型细胞中也发现这种积累的中间转录物和其他更长的转录物是低丰度RNA，从而可以鉴定出将原始转录物转化为最终产物所必需的加工事件。我们的数据直接证明Rpm1r是从位于tRNA met f基因上游的启动子与其底物tRNA met f和tRNAPro一起转录的，并表明一部分也源自位于tRNA met f基因和RPM1之间的第二个启动子。。我们测试了前体积累的可能性，因为RNase P缺乏会阻止下游tRNAPro的去除。大的RPM1转录物仍在缺少该tRNA的菌株中积累。因此，无法处理共转录的tRNA不能解释前体积累表型。此外，带有突变RPM1基因的菌株也积聚了前体Rpm1r，表明这两个基因中的突变都可能导致相似的生物发生缺陷。提出了几种解释前体积累的模型。

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《Molecular and Cellular Biology》 |1996年第7期|共8页
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V Stribinskis; G J Gao; P Sulo; Y L Dang; N C Martin;
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1. Rpm2, the Protein Subunit of Mitochondrial RNase P in Saccharomyces cerevisiae, Also Has a Role in the Translation of Mitochondrially Encoded Subunits of Cytochrome c Oxidase [J] . Guo-Jian Gao, Nancy C. Martin, Steven R. Ellis, Genetics: A Periodical Record of Investigations Bearing on Heredity and Variation . 2001,第2期

机译：Rpm2，酿酒酵母中的线粒体RNase P的蛋白质亚基，在细胞色素c氧化酶的线粒体编码亚基的翻译中也有作用
2. Rpm2, the Protein Subunit of Mitochondrial RNase P in Saccharomyces cerevisiae, Also Has a Role in the Translation of Mitochondrially Encoded Subunits of Cytochrome c Oxidase [J] . Guo-Jian Gao, Nancy C. Martin, Steven R. Ellis, Genetics: A Periodical Record of Investigations Bearing on Heredity and Variation . 2001,第2期

机译：Rpm2，酿酒酵母中的线粒体RNase P的蛋白质亚基，在细胞色素c氧化酶的线粒体编码亚基的翻译中也有作用
3. Characterization of yeast mitochondrial RNase P: an intact RNA subunit is not essential for activity in vitro [J] . Carol A. Wise, Margaret J. Hollingsworth, Michael J. Morales, Nucleic acids research . 1989,第17期

机译：酵母线粒体RNase P的表征：完整的RNA亚基对于体外活性不是必需的
4. Design, Synthesis and RNase H activity of Hemi-Gapmer Type Peptide Ribonucleic Acid (PRNA)-DNA chimeras for the RNase H mediated catalytic oligonucleotide therapeutics [C] . Masahito Inagaki, Daisuke Unabara, Ryohei Uematsu 日本化学会春季年会講演予稿集 . 2018

机译：半介距型肽核糖核糖核酸（PRNA）-DNA嵌合催化寡核苷酸治疗剂的设计，合成和RNase H活性
5. Characterization of pleiotropic pet mutants defective in regulation of mitochondrial respiration and mitochondrial protein synthesis. [D] . Jin, Can. 2001

机译：表征多效性宠物突变体在调节线粒体呼吸和线粒体蛋白质合成方面存在缺陷。
6. Yeast mitochondrial RNase P RNA synthesis is altered in an RNase P protein subunit mutant: insights into the biogenesis of a mitochondrial RNA-processing enzyme. [O] . V Stribinskis, G J Gao, P Sulo, 1996

机译：酵母线粒体RNase P RNA的合成在RNase P蛋白亚基突变体中发生了变化：洞察线粒体RNA加工酶的生物发生。
7. Yeast mitochondrial RNase P RNA synthesis is altered in an RNase P protein subunit mutant: insights into the biogenesis of a mitochondrial RNA-processing enzyme [O] . V Stribinskis, G J Gao, P Sulo, 1996

机译：酵母线粒体rNase p RNA合成在RNase P蛋白亚基突变体中改变：洞察线粒体RNA加工酶的生物发生

Yeast mitochondrial RNase P RNA synthesis is altered in an RNase P protein subunit mutant: insights into the biogenesis of a mitochondrial RNA-processing enzyme.

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