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首页> 外文期刊>Molecular and Cellular Biology >The FLP recombinase of the Saccharomyces cerevisiae 2 microns plasmid attaches covalently to DNA via a phosphotyrosyl linkage.
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The FLP recombinase of the Saccharomyces cerevisiae 2 microns plasmid attaches covalently to DNA via a phosphotyrosyl linkage.

机译：酿酒酵母（Saccharomyces cerevisiae）2微米质粒的FLP重组酶通过磷酸酪氨酰键与DNA共价连接。

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摘要

The FLP recombinase, encoded by the 2 micron plasmid of Saccharomyces cerevisiae, promotes efficient recombination in vivo and in vitro between its specific target sites (FLP sites). It was previously determined that FLP interacts with DNA sequences within its target site (B. J. Andrews, G. A. Proteau, L. G. Beatty, and P. D. Sadowski. Cell 40:795-803, 1985), generates a single-stranded break on both DNA strands within the FLP site, and remains covalently attached to the 3' end of each break. We now show that the FLP protein is bound to the 3' side of each break by an O-phosphotyrosyl residue and that it appears that the same tyrosyl residue(s) is used to attach to either DNA strand within the FLP site.
机译：由酿酒酵母的2微米质粒编码的FLP重组酶可促进体内和体外在其特定靶位点（FLP位点）之间的有效重组。先前已确定FLP与目标位点内的DNA序列相互作用（BJ Andrews，GA Proteau，LG Beatty和PD Sadowski。Cell 40：795-803，1985），在FLP中的两条DNA链上产生单链断裂。 FLP位点，并保持共价连接至每个断裂的3'末端。我们现在显示，FLP蛋白通过O-磷酸酪氨酰残基与每个断裂的3'侧结合，并且似乎使用了相同的酪氨酰残基来连接FLP位点内的任一DNA链。

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