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首页> 外文期刊>Molecular and Cellular Biology >H-2Ld antigen encoded by a recombinant retrovirus genome is expressed on the surface of infected cells.
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H-2Ld antigen encoded by a recombinant retrovirus genome is expressed on the surface of infected cells.

机译：重组逆转录病毒基因组编码的H-2Ld抗原在感染细胞表面表达。

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摘要

A recombinant murine retrovirus was constructed which contains, within its genome, a truncated version of the gene encoding the murine H-2Ld major histocompatibility antigen. The H-2Ld gene, which was inserted 3' of the env splice acceptor site in the recombinant retrovirus MSV-neo, lacked the 5' promoter and TATA sequences and the 3' transcription termination and polyadenylate addition sites of the normal H-2Ld gene. Transfection of the MSV-neo/H-2Ld plasmid (pLTV-11) into Y-2 cells resulted in the production of the transmissible recombinant retrovirus LTV-11. Cells infected with LTV-11 virus were resistant to the eucaryotic antibiotic G418 and expressed H-2Ld on the cell surface. These infected cells contained a viral RNA species which possessed both the H-2Ld and the neomycin resistance gene sequences but did not contain significant levels of the smaller H-2Ld-specific mRNA. The H-2Ld antigen expressed on the surface of infected cells functioned as a target for cytolytic T cells specific for the H-2Ld antigen.
机译：构建了重组鼠逆转录病毒，其在其基因组内含有编码鼠H-2Ld主要组织相容性抗原的基因的截短形式。 H-2Ld基因插入重组逆转录病毒MSV-neo中env剪接受体位点的3'，缺少正常H-2Ld基因的5'启动子和TATA序列以及3'转录终止和聚腺苷酸添加位点。 MSV-neo / H-2Ld质粒（pLTV-11）转染到Y-2细胞中导致了可传播的重组逆转录病毒LTV-11的产生。感染LTV-11病毒的细胞对真核抗生素G418具有抗性，并在细胞表面表达H-2Ld。这些被感染的细胞含有病毒RNA，既具有H-2Ld和新霉素抗性基因序列，又不包含显着水平的较小的H-2Ld特异性mRNA。在感染细胞表面表达的H-2Ld抗原可作为特异性针对H-2Ld抗原的溶细胞性T细胞的靶标。

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