• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Molecular and Cellular Biology >Characterization of G1 transit induced by the mitogenic-oncogenic viral Ki-ras gene product.
【24h】

Characterization of G1 transit induced by the mitogenic-oncogenic viral Ki-ras gene product.

机译：有丝分裂致癌性病毒Ki-ras基因产物诱导的G1转运的特征。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

NRK rat kidney cells infected with a temperature-sensitive mutant of the Kirsten sarcoma virus (ts371) were transformed at 36 degrees C but were phenotypically nontransformed at 41 degrees C because of the abnormal thermolability of the oncogenic 21-kilodalton product of the viral Ki-ras gene. Thus tsK-NRK cells were rendered quiescent in a G0-G1 state by a 48-h incubation in serum-free medium at the nonpermissive, p21-inactivating temperature of 41 degrees C. The serum-starved cells could then be stimulated to transit G1 either as nontransformed cells by adding serum at 41 degrees C or as transformed cells by lowering the temperature to a p21-activating 36 degrees C. The viral p21 protein was as effective as serum in stimulating tsK-NRK cells to transit G1 and to start replicating DNA. While p21 effectively stimulated cells to transit G1 even in unconditioned, serum-free medium, they still needed cell-derived conditioning factors to subsequently divide. The p21 protein also enabled the cells to transit G1 in spite of an extracellular Ca2+ deficiency that inhibited the G1 transit of serum-stimulated cells. p21 activity was needed to stimulate both early and late G1 events. In contrast to serum, p21 did not stimulate total RNA or protein synthesis, but some RNA and protein synthesis must have been needed for the p21-driven G1 transit because it could be stopped by actinomycin D or cycloheximide.
机译：感染了Kirsten肉瘤病毒（ts371）的温度敏感突变体的NRK大鼠肾细胞在36摄氏度下转化，但由于病毒Ki-的21致千金尔顿产物的致癌性异常，因此在41摄氏度下未进行表型转化。 ras基因。因此，通过在无血清，p21失活温度为41摄氏度的无血清培养基中孵育48小时，可使tsK-NRK细胞在G0-G1状态下静止。然后可以刺激血清饥饿的细胞通过G1通过在41摄氏度下添加血清作为未转化细胞，或者通过将温度降低至激活p21的36摄氏度作为转化细胞。病毒p21蛋白在刺激tsK-NRK细胞通过G1并开始复制方面与血清一样有效脱氧核糖核酸。尽管p21即使在无条件的无血清培养基中也能有效刺激细胞通过G1，但它们仍需要细胞衍生的条件因子才能分裂。尽管胞外Ca2 +缺乏抑制了血清刺激细胞的G1转运，但p21蛋白也使细胞能够转运G1。需要p21活性来刺激早期和晚期G1事件。与血清相反，p21不会刺激总RNA或蛋白质的合成，但是p21驱动的G1转运必须需要一些RNA和蛋白质的合成，因为它可能被放线菌素D或环己酰亚胺阻止。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号