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首页> 外文期刊>Yeast >Molecular characterization of Candida boidinii MIG1 and its role in the regulation of methanol-inducible gene expression.
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Molecular characterization of Candida boidinii MIG1 and its role in the regulation of methanol-inducible gene expression.

机译：Boidinii假丝酵母MIG1的分子特征及其在甲醇诱导的基因表达调控中的作用。

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摘要

Methanol-inducible gene promoters in methanol-utilizing yeasts are used in high-level heterologous gene expression systems. Generally, expression of methanol-inducible genes is completely repressed by the presence of glucose. In this study we identified the MIG1 gene in Candida boidinii, which encodes a homologue of the glucose repressor Mig1p of Saccharomyces cerevisiae. Disruption of the CbMIG1 gene had no growth effect on various carbon sources. Activation of the methanol-inducible AOD1 gene, which encodes alcohol oxidase, was increased in the early stage of methanol induction when cells of the CbMIG1-disrupted strain were transferred from glucose medium to methanol medium. Furthermore, CbMig1p tagged with yellow fluorescent protein was primarily localized in the nucleus of glucose-grown cells, but was diffuse in the cytosol of methanol-grown cells. This cytosolic diffusion in methanol-grown cells occurred in a CbMsn5p-dependent manner. These results suggest that CbMig1p is involved in negative regulation of methanol-inducible gene expression in C. boidinii. Copyright Copyright 2012 John Wiley & Sons, Ltd. Copyright Copyright 2012 John Wiley & Sons, Ltd.
机译：利用甲醇的酵母中的甲醇诱导型基因启动子被用于高级异源基因表达系统。通常，葡萄糖的存在会完全抑制甲醇诱导基因的表达。在这项研究中，我们确定了Bodinii假丝酵母中的MIG1基因，该基因编码酿酒酵母的葡萄糖阻遏物Mig1p的同源物。 CbMIG1基因的破坏对各种碳源均无生长作用。当CbMIG1破坏菌株的细胞从葡萄糖培养基转移到甲醇培养基时，在甲醇诱导的早期，编码乙醇氧化酶的甲醇诱导型AOD1基因的激活增加。此外，标记有黄色荧光蛋白的CbMig1p主要位于葡萄糖生长细胞的细胞核中，但分散在甲醇生长细胞的细胞质中。这种在甲醇生长的细胞中的胞质扩散是以CbMsn5p依赖的方式发生的。这些结果表明CbMig1p参与Boidinii甲醇诱导基因表达的负调控。版权所有©2012 John Wiley＆Sons，Ltd.。版权所有©2012 John Wiley＆Sons，Ltd.。

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