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首页> 外文期刊>Molecular and Cellular Biology >The adenine-thymine domain of the simian virus 40 core origin directs DNA bending and coordinately regulates DNA replication.
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The adenine-thymine domain of the simian virus 40 core origin directs DNA bending and coordinately regulates DNA replication.

机译：猿猴病毒40核心来源的腺嘌呤胸腺嘧啶域指导DNA弯曲并协调调节DNA复制。

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摘要

The simian virus 40 origin of replication contains a 20-base-pair adenine-thymine-rich segment with the sequence 5'-TGCATAAATAAAAAAAATTA-3'. The continuous tract of eight adenines is highly conserved among polyomaviruses. We used single-base substitutions to map structural and functional features of this DNA. Mutations in the AAA and AAAAAAAATT sequences significantly reduce DNA replication and thus identify two sequence-specific functional domains or a single domain with two parts. The AAAAAAAATT sequence also determines a DNA conformation that is characteristic of DNA bending. Single-base mutations in this domain change the degree of net bending, presumably by altering the length and location of the bending sequence. Thus, DNA bending in the correct conformation and location may be a structural signal for replication in polyomavirus origins and perhaps in other origins of replication with consecutive runs of adenines. The first five base pairs (TGCAT) of the 20-base-pair segment and the T between the AAA and AAAAAAAATT domains serve a sequence-independent function that may establish proper spacing within the core origin.
机译：猿猴病毒40的复制起点包含一个富含20个碱基对的腺嘌呤-胸腺嘧啶片段，其序列为5'-TGCATAAATAAAAAAAATTA-3'。在多瘤病毒中，八个腺嘌呤的连续片段高度保守。我们使用单碱基取代来绘制该DNA的结构和功能特征。 AAA和AAAAAAAATT序列中的突变会显着减少DNA复制，从而鉴定出两个序列特异性功能域或具有两个部分的单个域。 AAAAAAAATT序列还确定了DNA弯曲特征的DNA构象。此域中的单碱基突变可能会改变净弯曲的程度，大概是通过改变弯曲序列的长度和位置来实现的。因此，弯曲成正确构象和位置的DNA可能是在多瘤病毒起源中以及可能在腺嘌呤连续运行的其他复制起源中复制的结构信号。 20个碱基对片段的前五个碱基对（TGCAT）以及AAA和AAAAAAAATT域之间的T发挥序列无关功能，可在核心起始点内建立适当的间隔。

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