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首页> 外文期刊>Molecular and Cellular Biology >Induction of specific transcription by RNA polymerase III in transformed cells.
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Induction of specific transcription by RNA polymerase III in transformed cells.

机译：RNA聚合酶III在转化细胞中诱导特异性转录。

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摘要

RNA polymerase III (pol III) transcripts of the highly repeated mouse B2 gene family are increased in many oncogenically transformed murine cell lines. In cells transformed by simian virus 40, the small, cytoplasmic B2 RNAs are present at 20-fold-higher levels than in normal cells (M. R. D. Scott, K. Westphal, and P. W. J. Rigby, Cell 34:557-567, 1983; K. Singh, M. Carey, S. Saragosti, and M. Botchan, Nature [London] 314:553-556). We found that transcripts of the highly repeated B1 gene family are also increased 20-fold upon simian virus 40 transformation and showed that these RNAs result from pol III transcription. In contrast, transcripts from less highly repeated pol III templates such as the 5S, 7SL, 7SK, 4.5SI, tRNAMet, and tRNAPro genes are unaffected. The expression of the B2 RNAs in isolated nuclei shows that the augmentation is due mainly to an increased rate of transcription by pol III. There is thus specific transformation-inducible pol III transcription. We developed an in vitro transcription assay which utilizes genomic DNA as a template to study the transcription of all members of a repetitive gene family in their native context. This assay reproduces the low cytoplasmic levels of B1 compared with B2 RNAs suggesting that this ratio is dictated by intrinsic signals in the DNA.
机译：在许多致癌转化的鼠细胞系中，高度重复的小鼠B2基因家族的RNA聚合酶III（pol III）转录本增加。在猿猴病毒40转化的细胞中，小的细胞质B2 RNA的存在量比正常细胞中高20倍（MRD Scott，K.Westphal和PWJ Rigby，Cell 34：557-567，1983; K. Singh，M。Carey，S。Saragosti和M.Botchan，自然[伦敦] 314：553-556）。我们发现高度重复的B1基因家族的转录本在猿猴病毒40转化后也增加了20倍，并显示这些RNA是由pol III转录产生的。相反，来自重复程度较低的pol III模板（如5S，7SL，7SK，4.5SI，tRNAMet和tRNAPro基因）的转录本不受影响。 B2 RNA在分离核中的表达表明，这种增加主要是由于pol III转录速率的提高。因此，存在特定的转化诱导型pol III转录。我们开发了一种体外转录测定法，该方法利用基因组DNA作为模板来研究重复基因家族所有成员在其天然环境中的转录。与B2 RNA相比，该测定法重现了B1的低细胞质水平，表明该比例由DNA中的固有信号决定。

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