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The intron-containing gene for yeast profilin (PFY) encodes a vital function.

机译:酵母profilin(PFY)的含内含子基因编码重要功能。

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The gene coding for profilin (PFY), an actin-binding protein, occurs as a single copy in the haploid genome of Saccharomyces cerevisiae and is required for spore germination and cell viability. Displacement of one gene copy in a diploid cell by a nonfunctional allele is recessively lethal: tetrad analysis yields only two viable spores per ascus. The PFY gene maps on chromosome XV and is linked to the ADE2 marker. The primary transcript of about 1,000 bases contains an intron of 209 bases and is spliced into a messenger of about 750 bases. The intron was identified by comparison with a cDNA clone, which also revealed the 3' end of the transcript. The 5' end of the mRNA was mapped by primer elongation. The gene is transcribed constitutively and has a coding capacity for a protein of 126 amino acids. The deduced molecular weight of
机译:编码肌动蛋白结合蛋白(profilin)(PFY)的基因在酿酒酵母的单倍体基因组中以单拷贝形式出现,是孢子萌发和细胞活力所必需的。非功能性等位基因在二倍体细胞中置换一个基因拷贝会导致致命性的死亡:四分体分析每个曲柄仅产生两个活孢子。 PFY基因位于XV染色体上,并与ADE2标记连锁。大约1,000个碱基的初级转录本包含一个209个碱基的内含子,并被剪接到一个大约750个碱基的信使中。通过与cDNA克隆比较来鉴定内含子,该cDNA克隆也揭示了转录物的3'末端。 mRNA的5'末端通过引物延伸来定位。该基因是组成型转录的,具有126个氨基酸的蛋白质的编码能力。推导的分子量

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