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首页> 外文期刊>Molecular and Cellular Biology >Chinese hamster ovary cell mutants with multiple glycosylation defects for production of glycoproteins with minimal carbohydrate heterogeneity.
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Chinese hamster ovary cell mutants with multiple glycosylation defects for production of glycoproteins with minimal carbohydrate heterogeneity.

机译：中国仓鼠卵巢细胞突变体，具有多个糖基化缺陷，可产生糖异质性最小的糖蛋白。

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摘要

The production of glycoproteins with carbohydrates of defined structure and minimal heterogeneity is important for functional studies of mammalian carbohydrates. To facilitate such studies, several Chinese hamster ovary mutants that carry between two and four glycosylation mutations were developed. All of the lines grew readily in culture despite the drastic simplification of their surface carbohydrates. Therefore, both endogenous glycoproteins and those introduced by transfection can be obtained with specifically tailored carbohydrates. The lectin resistance properties of the mutants showed that each line expresses a novel array of cell surface carbohydrates useful for identifying specific roles for carbohydrates in cellular interactions. In addition, they showed that the epistatic relationships among different glycosylation mutations are not entirely predictable, providing insight into the complexity of the carbohydrate structures at the Chinese hamster ovary cell surface.
机译：具有确定结构和最小异质性的碳水化合物的糖蛋白的生产对于哺乳动物碳水化合物的功能研究很重要。为促进此类研究，开发了几种携带两个至四个糖基化突变的中国仓鼠卵巢突变体。尽管大大简化了其表面碳水化合物，所有品系在培养中仍很容易生长。因此，内源糖蛋白和通过转染引入的糖蛋白均可通过专门定制的碳水化合物获得。突变体的凝集素抗性特性表明，每条品系表达一种新颖的细胞表面碳水化合物阵列，可用于鉴定碳水化合物在细胞相互作用中的特定作用。此外，他们表明，不同糖基化突变之间的上位性关系不是完全可预测的，从而洞察了中国仓鼠卵巢细胞表面碳水化合物结构的复杂性。

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