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High-resolution footprints of the DNA-binding domain of Epstein-Barr virus nuclear antigen 1.

机译:EB病毒核抗原1的DNA结合结构域的高分辨率印迹。

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The DNA-binding domain of Epstein-Barr virus nuclear antigen 1 was found by hydroxyl radical footprinting to protect backbone positions on one side of its DNA-binding site. The guanines contacted in the major groove by the DNA-binding domain of Epstein-Barr virus nuclear antigen 1 were identified by methylation protection. No difference was found in the interaction of the DNA-binding domain of Epstein-Barr virus nuclear antigen 1 with tandemly repeated and overlapping binding sites.
机译:通过羟基自由基足迹法发现爱泼斯坦-巴尔病毒核抗原1的DNA结合结构域,以保护其DNA结合位点一侧的骨架位置。通过甲基化保护来鉴定在大沟中与爱泼斯坦-巴尔病毒核抗原1的DNA结合结构域接触的鸟嘌呤。在爱泼斯坦-巴尔病毒核抗原1的DNA结合结构域与串联重复和重叠的结合位点的相互作用中未发现差异。

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