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首页> 外文期刊>Molecular and Cellular Biology >Regulation of the chicken embryonic myosin light-chain (L23) gene: existence of a common regulatory element shared by myosin alkali light-chain genes.
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Regulation of the chicken embryonic myosin light-chain (L23) gene: existence of a common regulatory element shared by myosin alkali light-chain genes.

机译：鸡胚胎肌球蛋白轻链（L23）基因的调节：存在肌球蛋白碱性轻链基因共有的共同调节元件。

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摘要

The transcriptional regulation of the chicken myosin alkali light-chain (MLC) L23 gene was analyzed. Two different types of cis-regulatory regions were identified: one was a silencerlike region located between 3.7 and 2.7 kilobases upstream of the mRNA initiation site, and the other was essential for the expression of L23 in skeletal muscle cells and was located between 106 and 91 base pairs upstream of the cap site. This 16-base-pair cis-acting element was designated as the MLC box since it is well conserved in various muscle-specific MLC promoter regions. The activity of the MLC box showed tissue specificity. To analyze the relationship between the nucleotide sequence and the activity of the MLC box precisely, mutation analysis was performed. The 16-base-pair sequence was indispensable for the active transcription of L23 gene, and the MLC box could function in either orientation. The inverted sequence of the MLC box was similar to the sequence of the alpha-actin CArG box. By using a gel mobility retardation assay, the nuclear protein(s) that binds to both MLC box and CArG box was detected with nuclear extract prepared from chicken embryonic breast muscle. These observations imply that a common factor regulates the coordinate expression of these contractile proteins in muscle differentiation.
机译：分析了鸡肌球蛋白碱轻链（MLC）L23基因的转录调控。鉴定出两种不同类型的顺式调节区域：一种是位于mRNA起始位点上游3.7至2.7kb之间的沉默子样区域，另一种是在骨骼肌细胞中表达L23所必需的，位于106至91之间。帽位点上游的碱基对。这个16个碱基对的顺式作用元件被指定为MLC盒，因为它在各种肌肉特异性MLC启动子区域中都非常保守。 MLC盒的活性显示出组织特异性。为了精确分析核苷酸序列与MLC盒活性之间的关系，进行了突变分析。 16个碱基对的序列对于L23基因的有效转录是必不可少的，并且MLC盒可以在任一方向上起作用。 MLC盒的反向序列类似于α-肌动蛋白CArG盒的序列。通过使用凝胶迁移阻滞测定法，用从鸡胚胸肌制备的核提取物检测了与MLC box和CArG box结合的核蛋白。这些观察结果暗示一个共同因素调节这些收缩蛋白在肌肉分化中的协调表达。

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