Identification and structure of four yeast genes (SLY) that are able to suppress the functional loss of YPT1, a member of the RAS superfamily.

C Dascher; R Ossig; D Gallwitz; H D Schmitt

首页> 外文期刊>Molecular and Cellular Biology >Identification and structure of four yeast genes (SLY) that are able to suppress the functional loss of YPT1, a member of the RAS superfamily.

【24h】

Identification and structure of four yeast genes (SLY) that are able to suppress the functional loss of YPT1, a member of the RAS superfamily.

机译：四个酵母基因（SLY）的鉴定和结构，它们能够抑制RAS超家族成员YPT1的功能丧失。

获取原文

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

In Saccharomyces cerevisiae, the GTP-binding Ypt1 protein (Ypt1p) is essential for endoplasmic reticulum-to-Golgi protein transport. By exploiting a GAL10-YPT1 fusion to regulate YPT1 expression, three multicopy suppressors, SLY2, SLY12, and SLY41, and a single-copy suppressor, SLY1-20, that allowed YPT1-independent growth were isolated. Wild-type Sly1p is hydrophilic, is essential for cell viability, and differs from Sly1-20p by a single amino acid. SLY2 and SLY12 encode proteins with hydrophobic tails similar to synaptobrevins, integral membrane proteins of synaptic vesicles in higher eucaryotes. Sly41p is hydrophobic and exhibits sequence similarities with the chloroplast phosphate translocator. SLY12 but not SLY41 is an essential gene. The SLY2 null mutant is cold and heat sensitive. The SLY gene products may comprise elements of the protein transport machinery.

机译：在酿酒酵母中，GTP结合Ypt1蛋白（Ypt1p）对于内质网到高尔基体蛋白运输至关重要。通过利用GAL10-YPT1融合物调节YPT1的表达，分离了三种多拷贝抑制剂SLY2，SLY12和SLY41，以及单拷贝抑制剂SLY1-20，它们可以独立于YPT1生长。野生型Sly1p是亲水性的，对于细胞活力至关重要，并且与Sly1-20p的区别仅在于一个氨基酸。 SLY2和SLY12编码具有疏水尾巴的蛋白，类似于高真核生物中突触小泡的整合膜蛋白，即突触小泡蛋白。 Sly41p是疏水的，并且与叶绿体磷酸酯转运蛋白表现出序列相似性。 SLY12而非SLY41是必需基因。 SLY2无效突变体对冷热敏感。 SLY基因产物可以包含蛋白质转运机制的元件。

著录项

来源
《Molecular and Cellular Biology》 |1991年第2期|共14页
作者
C Dascher; R Ossig; D Gallwitz; H D Schmitt;
展开▼
作者单位

展开▼
收录信息
原文格式 PDF
正文语种
中图分类细胞生物学;
关键词

相似文献

外文文献
中文文献
专利

1. The yeast SLY gene products, suppressors of defects in the essential GTP-binding Ypt1 protein, may act in endoplasmic reticulum-to-Golgi transport. [J] . R Ossig, C Dascher, H H Trepte, Molecular and Cellular Biology . 1991,第6期

机译：酵母SLY基因产物是必需GTP结合Ypt1蛋白缺陷的抑制因子，可能在内质网到高尔基体的运输中起作用。
2. Multicopy tRNA genes functionally suppress mutations in yeast eIF-2 alpha kinase GCN2: evidence for separate pathways coupling GCN4 expression to unchanged tRNA. [J] . C R Vazquez de Aldana, R C Wek, P S Segundo, Molecular and Cellular Biology . 1994,第12期

机译：多拷贝tRNA基因功能上抑制酵母eIF-2α激酶GCN2中的突变：将GCN4表达与不变的tRNA偶联的独立途径的证据。
3. Identification of partial loss of function p53 gene mutations utilizing a yeast-based functional assay. [J] . Kovvali GK, Mehta B, Epstein CB, Nucleic Acids Research . 2001,第5期

机译：利用基于酵母的功能测定鉴定功能性p53基因突变的部分损失。
4. Identification and Functional Characterization of Pheromone Induced Fus3 Specific Phosphorylation Sites of the Yeast 26S Proteasome by Quantitative Mass Spectrometry [C] . Robyn Kaake, Lan Huang American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics . 2010

机译：通过定量质谱法鉴定和功能表征酵母26S蛋白酶体的FUS3特异性磷酸化位点
5. Identification and characterization of TAPIR, a novelc-Rel target gene that can suppress NF-kappaB-mediated gene activation. [D] . Lizzul, Paul F. 2002

机译：TAPIR的鉴定和表征，TAPIR是一种可抑制NF-κB介导的基因激活的新型c-Rel靶基因。
6. Identification and structure of four yeast genes (SLY) that are able to suppress the functional loss of YPT1 a member of the RAS superfamily. [O] . C Dascher, R Ossig, D Gallwitz, 1991

机译：能够抑制RAS超家族成员YPT1功能丧失的四个酵母基因（SLY）的鉴定和结构。
7. Identification and structure of four yeast genes (SLY) that are able to suppress the functional loss of YPT1, a member of the RAS superfamily. [O] . Dascher, C, Ossig, R, Gallwitz, D, 1991

机译：能够抑制RAS超家族成员YPT1功能丧失的四个酵母基因（SLY）的鉴定和结构。

Identification and structure of four yeast genes (SLY) that are able to suppress the functional loss of YPT1, a member of the RAS superfamily.

摘要

著录项

相似文献

相关主题

期刊订阅