Expression of antisense RNA against initiation factor eIF-4E mRNA in HeLa cells results in lengthened cell division times, diminished translation rates, and reduced levels of both eIF-4E and the p220 component of eIF-4F.

A De Benedetti; S Joshi-Barve; C Rinker-Schaeffer; R E Rhoads

首页> 外文期刊>Molecular and Cellular Biology >Expression of antisense RNA against initiation factor eIF-4E mRNA in HeLa cells results in lengthened cell division times, diminished translation rates, and reduced levels of both eIF-4E and the p220 component of eIF-4F.

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Expression of antisense RNA against initiation factor eIF-4E mRNA in HeLa cells results in lengthened cell division times, diminished translation rates, and reduced levels of both eIF-4E and the p220 component of eIF-4F.

机译：HeLa细胞中针对起始因子eIF-4E mRNA的反义RNA的表达导致细胞分裂时间延长，翻译速率降低以及eIF-4E和eIF-4F的p220组分水平降低。

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HeLa cells were transformed to express antisense RNA against initiation factor eIF-4E mRNA from an inducible promoter. In the absence of inducer, these cells (AS cells) were morphologically similar to control cells but grew four- to sevenfold more slowly. Induction of antisense RNA production was lethal. Both eIF-4E mRNA and protein levels were reduced in proportion to the degree of antisense RNA expression, as were the rates of protein synthesis in vivo and in vitro. Polysomes were disaggregated with a concomitant increase in ribosomal subunits. Translation in vitro was restored by addition of the initiation factor complex eIF-4F but not by eIF-4E alone. Immunological analysis revealed that the p220 component of eIF-4F was decreased in extracts of AS cells and undetectable in AS cells treated with inducer, suggesting that p220 and eIF-4E levels are coordinately regulated. eIF-4A, another component of eIF-4F, was unaltered.

机译：转化HeLa细胞以表达针对来自诱导型启动子的起始因子eIF-4E mRNA的反义RNA。在没有诱导剂的情况下，这些细胞（AS细胞）在形态上与对照细胞相似，但生长速度却慢了四到七倍。反义RNA产生的诱导是致命的。 eIF-4E mRNA和蛋白质水平均与反义RNA表达的程度成正比，体内和体外蛋白质的合成率也有所降低。多核糖体被分解，伴随着核糖体亚基的增加。通过添加起始因子复合物eIF-4F可以恢复体外翻译，但不能单独添加eIF-4E。免疫学分析显示，eIF-4F的p220成分在AS细胞的提取物中减少，而在诱导剂处理的AS细胞中则无法检测到，这表明p220和eIF-4E的水平受到协调调节。 eIF-4F的另一个组件eIF-4A保持不变。

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《Molecular and Cellular Biology》 |1991年第11期|共11页
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A De Benedetti; S Joshi-Barve; C Rinker-Schaeffer; R E Rhoads;
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中图分类细胞生物学;
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6. Expression of antisense RNA against initiation factor eIF-4E mRNA in HeLa cells results in lengthened cell division times diminished translation rates and reduced levels of both eIF-4E and the p220 component of eIF-4F. [O] . A De Benedetti, S Joshi-Barve, C Rinker-Schaeffer, 1991

机译：HeLa细胞中针对起始因子eIF-4E mRNA的反义RNA的表达导致细胞分裂时间延长翻译速率降低以及eIF-4E和eIF-4F的p220组分水平降低。
7. Expression of antisense RNA against initiation factor eIF-4E mRNA in HeLa cells results in lengthened cell division times, diminished translation rates, and reduced levels of both eIF-4E and the p220 component of eIF-4F. [O] . De Benedetti, A, Joshi-Barve, S, Rinker-Schaeffer, C, 1991

机译：HeLa细胞中针对起始因子eIF-4E mRNA的反义RNA的表达导致细胞分裂时间延长，翻译速率降低以及eIF-4E和eIF-4F的p220组分水平降低。

Expression of antisense RNA against initiation factor eIF-4E mRNA in HeLa cells results in lengthened cell division times, diminished translation rates, and reduced levels of both eIF-4E and the p220 component of eIF-4F.

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