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首页> 外文期刊>Molecular and Cellular Biology >Translation initiation in Drosophila melanogaster is reduced by mutations upstream of the AUG initiator codon.
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Translation initiation in Drosophila melanogaster is reduced by mutations upstream of the AUG initiator codon.

机译：果蝇黑腹果蝇的翻译起始通过AUG起始密码子上游的突变而减少。

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摘要

The importance to in vivo translation of sequences immediately upstream of the Drosophila alcohol dehydrogenase (Adh) start codon was examined at two developmental stages. Mutations were introduced into the Adh gene in vitro, and the mutant gene was inserted into the genome via germ line transformation. An A-to-T substitution at the -3 position did not affect relative translation rates of the ADH protein at the second-instar larval stage but resulted in a 2.4-fold drop in translation of ADH at the adult stage. A second mutant gene, containing five mutations in the region -1 to -9, was designed to completely block translation initiation. However, transformant lines bearing these mutations still exhibit detectable ADH, albeit at substantially reduced levels. The average fold reduction at the second-instar larval stage was 5.9, while at the adult stage a 12.5-fold reduction was observed.
机译：在两个发育阶段检查了果蝇酒精脱氢酶（Adh）起始密码子上游序列对体内翻译的重要性。在体外将突变引入Adh基因，并通过种系转化将突变基因插入基因组。在第二龄幼虫阶段，在-3位置的A到T取代不会影响ADH蛋白的相对翻译速率，但在成年期导致ADH的翻译下降2.4倍。第二个突变基因在区域-1到-9之间包含五个突变，旨在完全阻断翻译起始。但是，带有这些突变的转化株尽管水平明显降低，仍显示出可检测的ADH。在第二龄幼虫阶段平均减少了5.9倍，而在成年幼虫阶段减少了12.5倍。

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