• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Molecular and Cellular Biology >Antibodies specific for the human retinoblastoma protein identify a family of related polypeptides.
【24h】

Antibodies specific for the human retinoblastoma protein identify a family of related polypeptides.

机译：对人类视网膜母细胞瘤蛋白具有特异性的抗体可鉴定相关多肽家族。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Even though the retinoblastoma gene is one of the best-studied tumor suppressor genes, little is known about its functional role. Like all tumor suppressor gene products, the retinoblastoma protein (pRB) is thought to inhibit some aspect of cell proliferation. It also appears to be a cellular target of several DNA tumor virus-transforming proteins, such as adenovirus E1A, human papillomavirus E7, or simian virus 40 large T antigen. To help in the analysis of pRB, we have prepared a new set of anti-human pRB monoclonal antibodies. In addition to being useful reagents for the study of human pRB, these antibodies display several unexpected properties. They can be used to distinguish different subsets of the pRBs on the basis of their phosphorylation states. Some are able to recognize pRB homologs in other species, including mice, chickens, and members of the genus Xenopus. In addition, some of these antibodies can bind directly to other cellular proteins that, like pRB, were originally identified through their association with adenovirus E1A. These immunologically cross-reactive proteins include the p107 and p300 proteins, and their recognition by antibodies raised against pRB suggests that several members of the E1A-targeted cellular proteins form a structurally and functionally related family.
机译：尽管视网膜母细胞瘤基因是研究最多的抑癌基因之一，但对其功能的作用知之甚少。像所有肿瘤抑制基因产品一样，视网膜母细胞瘤蛋白（pRB）被认为可以抑制细胞增殖的某些方面。它也似乎是几种DNA肿瘤病毒转化蛋白的细胞靶标，例如腺病毒E1A，人乳头瘤病毒E7或猿猴病毒40大T抗原。为了帮助分析pRB，我们准备了一组新的抗人pRB单克隆抗体。这些抗体除了是用于研究人pRB的有用试剂外，还显示出一些出乎意料的特性。它们可以根据其磷酸化状态来区分pRB的不同子集。一些能够识别其他物种中的pRB同源物，包括小鼠，鸡和非洲爪蟾属的成员。此外，这些抗体中的一些可以直接结合其他细胞蛋白质，例如pRB，最初是通过与腺病毒E1A结合而鉴定的。这些免疫交叉反应蛋白包括p107和p300蛋白，它们被针对pRB的抗体识别表明，靶向E1A的细胞蛋白的几个成员形成了结构上和功能上相关的家族。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号