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首页> 外文期刊>Molecular and Cellular Biology >Tyrosine phosphorylation and activation of homologous protein kinases during oocyte maturation and mitogenic activation of fibroblasts.
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Tyrosine phosphorylation and activation of homologous protein kinases during oocyte maturation and mitogenic activation of fibroblasts.

机译：卵母细胞成熟过程中酪氨酸磷酸化和同源蛋白激酶的激活以及成纤维细胞的促有丝分裂激活。

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Meiotic maturation of Xenopus and sea star oocytes involves the activation of a number of protein-serine/threonine kinase activities, including a myelin basic protein (MBP) kinase. A 44-kDa MBP kinase (p44mpk) purified from mature sea star oocytes is shown here to be phosphorylated at tyrosine. Antiserum to purified sea star p44mpk was used to identify antigenically related proteins in Xenopus oocytes. Two tyrosine-phosphorylated 42-kDa proteins (p42) were detected with this antiserum in Xenopus eggs. Xenopus p42 chromatographs with MBP kinase activity on a Mono Q ion-exchange column. Tyrosine phosphorylation of Xenopus p42 approximately parallels MBP kinase activity during meiotic maturation. These results suggest that related MBP kinases are activated during meiotic maturation of Xenopus and sea star oocytes. Previous studies have suggested that Xenopus p42 is related to the mitogen-activated protein (MAP) kinases of culture mammalian cells. We have cloned a MAP kinase relative from a Xenopus ovary cDNA library and demonstrate that this clone encodes the Xenopus p42 that is tyrosine phosphorylated during oocyte maturation. Comparison of the sequences of Xenopus p42 and a rat MAP kinase (ERK1) and peptide sequences from sea star p44mpk indicates that these proteins are close relatives. The family members appear to be tyrosine phosphorylated, and activated, in different contexts, with the murine MAP kinase active during the transition from quiescence to the G1 stage of the mitotic cell cycle and the sea star and Xenopus kinases being active during M phase of the meiotic cell cycle.
机译：非洲爪蟾和海星卵母细胞的减数分裂成熟涉及许多蛋白质丝氨酸/苏氨酸激酶活性的激活，包括髓鞘碱性蛋白（MBP）激酶。从成熟的海星卵母细胞纯化得到的44 kDa MBP激酶（p44mpk）在酪氨酸处被磷酸化。针对纯化的海星p44mpk的抗血清用于鉴定爪蟾卵母细胞中的抗原相关蛋白。用这种抗血清在非洲爪蟾卵中检测到两种酪氨酸磷酸化的42 kDa蛋白（p42）。 Xenopus p42色谱在Mono Q离子交换柱上具有MBP激酶活性。非洲爪蟾p42的酪氨酸磷酸化与减数分裂成熟期间的MBP激酶活性大致平行​​。这些结果表明，相关的MBP激酶在非洲爪蟾和海星卵母细胞的减数分裂成熟过程中被激活。先前的研究表明，非洲爪蟾p42与培养哺乳动物细胞的促分裂原活化蛋白（MAP）激酶有关。我们已经从非洲爪蟾卵巢cDNA库中克隆了一个MAP激酶亲戚，并证明该克隆编码在卵母细胞成熟过程中被酪氨酸磷酸化的非洲爪蟾p42。爪蟾p42和大鼠MAP激酶（ERK1）的序列以及海星p44mpk的肽序列的比较表明，这些蛋白质是近亲。在不同的情况下，该家族成员似乎被酪氨酸磷酸化并被激活，在从有丝分裂细胞周期的静止期到G1期的转变中，鼠MAP激酶活跃，而在M期的M期中，海星和爪蟾激酶活跃。减数分裂细胞周期。

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