The gene for a novel human lamin maps at a highly transcribed locus of chromosome 19 which replicates at the onset of S-phase.

G Biamonti; M Giacca; G Perini; G Contreas; L Zentilin; F Weighardt; M Guerra; G Della Valle; S Saccone; S Riva

首页> 外文期刊>Molecular and Cellular Biology >The gene for a novel human lamin maps at a highly transcribed locus of chromosome 19 which replicates at the onset of S-phase.

【24h】

The gene for a novel human lamin maps at a highly transcribed locus of chromosome 19 which replicates at the onset of S-phase.

机译：新型人类lamin的基因定位在19号染色体的高度转录位点，该位点在S期开始时复制。

获取原文

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

A previously described human DNA fragment which is replicated early in S-phase of HL-60 cell DNA (C. Tribioli, G. Biamonti, M. Giacca, M. Colonna, S. Riva, and A. Falaschi, Nucleic Acids Res. 15:10211-10232, 1987) was used to screen a genomic library in lambda Ch28. A clone which contained a 13.7-kb insert (L30E) found to code for several transcripts was isolated. The transcription of L30E DNA exhibited a complex pattern and a tissue-specific and proliferation-dependent type of regulation. The data were consistent with two tandemly arranged transcription units, the 3' end of one separated from the 5' end of the other by a sequence of about 600 bp containing an active promoter. The isolation and sequencing of L30E-specific cDNAs permitted identification of two genes, one of which encoded a B-type human lamin (analogous to mouse lamin B2). L30E DNA was mapped by in situ hybridization at the G-negative subtelomeric band p13.3 of chromosome 19. Interestingly, in synchronized HL-60 cells, L30E DNA is replicated in the first minute of S-phase. Replication of the lamin gene early in S-phase may reflect a coupling between early replication and transcription of genes for S-phase-specific proteins such as lamins.

机译：先前描述的人类DNA片段，可在HL-60细胞DNA的S期早期复制（C.Tribioli，G.Biamonti，M.Giacca，M.Colonna，S.Riva和A.Falaschi，Nucleic Acids Res。 15：10211-10232，1987）用来筛选λCh28中的基因组文库。分离出含有13.7kb插入片段（L30E）的克隆，发现该克隆编码几种转录物。 L30E DNA的转录表现出复杂的模式以及组织特异性和增殖依赖性的调控类型。数据与两个串联排列的转录单元一致，一个的3'端与另一个的5'端隔开约600 bp的含有活性启动子的序列。 L30E特异性cDNA的分离和测序允许鉴定两个基因，其中一个编码B型人层粘连蛋白（类似于小鼠层粘连蛋白B2）。 L30E DNA通过原位杂交在19号染色体的G负亚端粒带p13.3定位。有趣的是，在同步化的HL-60细胞中，L30E DNA在S期的第一分钟被复制。 Lamin基因在S期早期的复制可能反映了S期特异性蛋白质（如lamin）的基因的早期复制与转录之间的耦合。

著录项

来源
《Molecular and Cellular Biology》 |1992年第8期|共8页
作者
G Biamonti; M Giacca; G Perini; G Contreas; L Zentilin; F Weighardt; M Guerra; G Della Valle; S Saccone; S Riva;
展开▼
作者单位

展开▼
收录信息
原文格式 PDF
正文语种
中图分类细胞生物学;
关键词

相似文献

外文文献
中文文献
专利

1. Identification and mapping of nuclear matrix-attachment regions in a one megabase locus of human chromosome 19q13.12: long-range correlation of S/MARs and gene positions. [J] . Chernov IgorP, Akopov SB, Nikolaev LG, Journal of cellular biochemistry. . 2002,第3期

机译：人类染色体19q13.12的一个1兆碱基基因座中核基质附着区域的鉴定和作图：S / MAR与基因位置的长期相关性。
2. Amplified dihydrofolate reductase genes are located in chromosome regions containing DNA that replicates during the first half of S-phase. [J] . R E Kellems, M E Harper, L M Smith Journal of cell biology . 1982,第2期

机译：扩增的二氢叶酸还原酶基因位于含有在S期前半段复制的DNA的染色体区域。
3. Amplified dihydrofolate reductase genes are located in chromosome regions containing DNA that replicates during the first half of S-phase. [J] . R E Kellems, M E Harper, L M Smith Journal of cell biology . 1982,第2期

机译：扩增的二氢叶酸还原酶基因位于含有在S期前半段复制的DNA的染色体区域。
4. Homozygosity Mapping of a Second Gene Locus for Hereditary Combined Deficiency of Witamin K-Dependent Clotting Factors (FMFD) to Chromosome 16 [C] . A. Fregin, S. Rost, W. Wolz, Hemophilie-Symposion . 2004

机译：用于血液磷酸血栓缺乏的第二基因遗迹的纯合理映射到染色体16
5. Development of a map-based cloning system in Sorghum bicolor: 1. Isolation of megabase-size DNA and construction of a bacterial artificial chromosome library and 2. Genetic and physical mapping of the 5S rDNA locus. [D] . Woo, Sung-Sick. 1996

机译：双色高粱基于图谱的克隆系统的开发：1.分离碱基大小庞大的DNA和构建细菌人工染色体文库，以及2. 5S rDNA基因座的遗传和物理作图。
6. The gene for a novel human lamin maps at a highly transcribed locus of chromosome 19 which replicates at the onset of S-phase. [O] . G Biamonti, M Giacca, G Perini, 1992

机译：新型人类lamin的基因定位在19号染色体的高度转录位点该位点在S期开始时复制。
7. The gene for a novel human lamin maps at a highly transcribed locus of chromosome 19 which replicates at the onset of S-phase. [O] . Biamonti, G, Giacca, M, Perini, G, 1992

机译：新型人类lamin的基因定位在19号染色体的高度转录位点，该位点在S期开始时复制。

The gene for a novel human lamin maps at a highly transcribed locus of chromosome 19 which replicates at the onset of S-phase.

摘要

著录项

相似文献

相关主题

期刊订阅