Zinc fingers and other domains cooperate in binding of Drosophila sry beta and delta proteins at specific chromosomal sites.

S Noselli; F Payre; A Vincent

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Zinc fingers and other domains cooperate in binding of Drosophila sry beta and delta proteins at specific chromosomal sites.

机译：锌指和其他域在特定的染色体位点上结合果蝇的β和δ蛋白。

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The closely related Drosophila serendipity (sry) beta and delta zinc finger proteins display consensus in vitro DNA recognition sequences differing by 4 of 13 nucleotide positions and bind in vivo to distinct sets of sites on polytene chromosomes. We compared the pattern of in vivo chromosomal binding of deleted forms of the sry delta protein fused to beta-galactosidase and expressed in Drosophila transgenic lines. Results show that the carboxy-terminal DNA-binding finger domain is required and sufficient for binding at specific chromosomal sites but that this binding does not nearly reproduce the wild-type pattern. An NH2-terminal domain of the sry delta protein is essential to its specificity of in vivo interaction with chromatin. In vitro and in vivo experiments using reciprocal finger swap between the sry beta and delta proteins suggest that the in vivo specificity is dependent on selective protein-protein contacts at defined chromosomal sites, in addition to DNA specific recognition.

机译：密切相关的果蝇偶然性（sry）β和δ锌指蛋白在13个核苷酸位置中有4个显示出一致的体外DNA识别序列，并在体内与多态染色体上不同的位点结合。我们比较了与β-半乳糖苷酶融合并在果蝇转基因株系中表达的缺失形式的sryδ蛋白的体内染色体结合模式。结果表明，羧基末端DNA结合指结构域是必需的，并且对于在特定染色体位点的结合是足够的，但是这种结合几乎不能复制野生型模式。 sryδ蛋白的NH2末端结构域对其在体内与染色质相互作用的特异性至关重要。使用sry beta蛋白和δ蛋白之间的相互手指互换的体外和体内实验表明，除了DNA特异性识别外，体内特异性还取决于在定义的染色体位点的选择性蛋白质-蛋白质接触。

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《Molecular and Cellular Biology》 |1992年第2期|共10页
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S Noselli; F Payre; A Vincent;
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1. Identification of the Drosophila interband-specific protein Z4 as a DNA-binding zinc-finger protein determining chromosomal structure. [J] . Eggert H, Gortchakov A, Saumweber H Journal of Cell Science . 2004,第Pta18期

机译：果蝇带间特异性蛋白Z4的鉴定为DNA结合锌指蛋白，可确定染色体结构。
2. Identification of the Drosophila interband-specific protein Z4 as a DNA-binding zinc-finger protein determining chromosomal structure [J] . Eggert H, Gortchakov A, Saumweber H Journal of Cell Science . 2004,第18期

机译：果蝇带间特异性蛋白Z4作为DNA结合锌指蛋白的确定染色体结构的鉴定。
3. Zinc finger protein binding to DNA: an energy perspective using molecular dynamics simulation and free energy calculations on mutants of both zinc finger domains and their specific DNA bases [J] . Hamed Mazen Y., Arya Gaurav Journal of Biomolecular Structure and Dynamics . 2016,第5a6期

机译：锌指蛋白与DNA的结合：使用分子动力学模拟和自由能计算的锌指结构域及其特定DNA碱基突变体的能量视角
4. Fuzzy sequence pattern matching in zinc finger domain proteins [C] . Chang, B.C.H., Halgamuge, IFSA World Congress and 20th NAFIPS International Conference, 2001. Joint 9th . 2001

机译：锌指结构域蛋白中的模糊序列模式匹配
5. Metal-binding properties of the zinc fingers in metal responsive element-binding transcription factor-1 and design of single-stranded nucleic acid binding proteins using nucleocapsid-like CCHC zinc-binding domains. [D] . Guerrerio, Anthony Lawrence, Jr. 2004

机译：锌指在金属反应元件结合转录因子-1中的金属结合特性，以及使用核衣壳样CCHC锌结合结构域设计单链核酸结合蛋白。
6. Zinc fingers and other domains cooperate in binding of Drosophila sry beta and delta proteins at specific chromosomal sites. [O] . S Noselli, F Payre, A Vincent 1992

机译：锌指和其他域在特定的染色体位点上结合果蝇的β和δ蛋白。
7. Zinc fingers and other domains cooperate in binding of Drosophila sry beta and delta proteins at specific chromosomal sites. [O] . Noselli, S, Payre, F, Vincent, A 1992

机译：锌指和其他域在特定的染色体位点上结合果蝇的β和δ蛋白。

Zinc fingers and other domains cooperate in binding of Drosophila sry beta and delta proteins at specific chromosomal sites.

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