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首页> 外文期刊>Molecular and Cellular Biology >Centromeres of the fission yeast Schizosaccharomyces pombe are highly variable genetic loci.
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Centromeres of the fission yeast Schizosaccharomyces pombe are highly variable genetic loci.

机译：裂变酵母粟酒裂殖酵母的着丝粒是高度可变的遗传基因座。

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Gross variations in the structure of the centromere of Schizosaccharomyces pombe chromosome III (cen3) were apparent following characterization of this centromeric DNA in strain Sp223 and comparison of the structure with that of cen3 in three other commonly used laboratory strains. Further differences in centromere structure were revealed when the structure of the centromere of S. pombe chromosome II (cen2) was compared among common laboratory strains and when the structures of cen2 and cen3 from our laboratory strains were compared with those reported from other laboratories. Differences observed in cen3 structure include variations in the arrangement of the centromeric K repeats and an inverted orientation of the conserved centromeric central core. In addition, we have identified two laboratory strains that contain a minimal cen2 repeat structure that lacks the tandem copies of the cen2-specific block of K-L-B-J repeats characteristic of Sp223 cen2. We have also determined that certain centromeric DNA structural motifs are relatively conserved among the four laboratory strains and eight additional wild-type S. pombe strains isolated from various food and beverage sources. We conclude that in S. pombe, as in higher eukaryotes, the centromere of a particular chromosome is not a defined genetic locus but can contain significant variability. However, the basic DNA structural motif of a central core immediately flanked by inverted repeats is a common parameter of the S. pombe centromere.
机译：在表征了Sp223菌株中着丝粒DNA并比较了其他三种常用实验室菌株中的cen3结构后，粟酒裂殖酵母III号染色体（cen3）的着丝粒的结构发生了明显的变化。当比较普通实验室菌株中的粟酒裂殖酵母II号染色体（cen2）的着丝粒结构，以及将我们实验室菌株中cen2和cen3的结构与其他实验室报告的结构进行比较时，揭示出着丝粒结构的进一步差异。在cen3结构中观察到的差异包括着丝粒K重复序列的排列变化和保守的着丝粒中心核的反向排列。此外，我们已经鉴定出两个实验室菌株，它们包含最小的cen2重复结构，而该结构缺少Sp223 cen2的K-L-B-J重复序列的cen2特异性嵌段的串联拷贝。我们还确定，从各种食品和饮料来源分离出的四种实验室菌株和八种其他野生型粟酒裂殖酵母菌株中，某些着丝粒DNA结构基序相对保守。我们得出的结论是，与高级真核生物一样，在粟酒裂殖酵母中，特定染色体的着丝粒不是定义的遗传位点，而是可以包含显着的变异性。然而，紧接在反向重复旁侧的中央核心的基本DNA结构基序是粟酒裂殖酵母着丝粒的共同参数。

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