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首页> 外文期刊>Molecular and Cellular Biology >PUB1 is a major nuclear and cytoplasmic polyadenylated RNA-binding protein in Saccharomyces cerevisiae.
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PUB1 is a major nuclear and cytoplasmic polyadenylated RNA-binding protein in Saccharomyces cerevisiae.

机译：PUB1是酿酒酵母中主要的核和细胞质聚腺苷酸化RNA结合蛋白。

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Proteins that directly associate with nuclear polyadenylated RNAs, or heterogeneous nuclear RNA-binding proteins (hnRNPs), and those that associate with cytoplasmic mRNAs, or mRNA-binding proteins (mRNPs), play important roles in regulating gene expression at the posttranscriptional level. Previous work with a variety of eukaryotic cells has demonstrated that hnRNPs are localized predominantly within the nucleus whereas mRNPs are cytoplasmic. While studying proteins associated with polyadenylated RNAs in Saccharomyces cerevisiae, we discovered an abundant polyuridylate-binding protein, PUB1, which appears to be both an hnRNP and an mRNP. PUB1 and PAB1, the polyadenylate tail-binding protein, are the two major proteins cross-linked by UV light to polyadenylated RNAs in vivo. The deduced primary structure of PUB1 indicates that it is a member of the ribonucleoprotein consensus sequence family of RNA-binding proteins and is structurally related to the human hnRNP M proteins. Even though the PUB1 protein is a major cellular polyadenylated RNA-binding protein, it is nonessential for cell growth. Indirect cellular immunofluorescence combined with digital image processing allowed a detailed comparison of the intracellular distributions of PUB1 and PAB1. While PAB1 is predominantly, and relatively uniformly, distributed within the cytoplasm, PUB1 is localized in a nonuniform pattern throughout both the nucleus and the cytoplasm. The cytoplasmic distribution of PUB1 is considerably more discontinuous than that of PAB1. Furthermore, sucrose gradient sedimentation analysis demonstrates that PAB1 cofractionates with polyribosomes whereas PUB1 does not. These results suggest that PUB1 is both an hnRNP and an mRNP and that it may be stably bound to a translationally inactive subpopulation of mRNAs within the cytoplasm.
机译：与核聚腺苷酸化的RNA或异质核RNA结合蛋白（hnRNPs）直接缔合的蛋白，以及与细胞质mRNA或mRNA结合蛋白（mRNPs）缔合的蛋白在转录后水平上调节基因表达中发挥着重要作用。先前对各种真核细胞的研究表明，hnRNPs主要位于细胞核内，而mRNPs是胞质的。在研究酿酒酵母中与聚腺苷酸化RNA相关的蛋白质时，我们发现了丰富的聚尿苷酸酯结合蛋白PUB1，它看起来既是hnRNP又是mRNP。聚腺苷酸尾结合蛋白PUB1和PAB1是在体内通过紫外线与聚腺苷酸化RNA交联的两个主要蛋白。推定的PUB1一级结构表明它是RNA结合蛋白的核糖核蛋白共有序列家族的成员，并且在结构上与人hnRNP M蛋白有关。即使PUB1蛋白是主要的细胞多聚腺苷酸RNA结合蛋白，它对细胞生长也不重要。间接细胞免疫荧光与数字图像处理相结合，可以对PUB1和PAB1的细胞内分布进行详细比较。虽然PAB1主要且相对均匀地分布在细胞质内，但PUB1分布在整个细胞核和细胞质中的位置不均匀。 PUB1的细胞质分布比PAB1的细胞质分布更加不连续。此外，蔗糖梯度沉降分析表明PAB1与多核糖体共馏，而PUB1没有。这些结果表明，PUB1既是hnRNP又是mRNP，并且可能与细胞质内mRNA的翻译无活性亚群稳定结合。

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