...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Molecular and Cellular Biology >Differential transformation of mammary epithelial cells by Wnt genes.
【24h】

Differential transformation of mammary epithelial cells by Wnt genes.

机译：Wnt基因差异转化乳腺上皮细胞。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The mouse Wnt family includes at least 10 genes that encode structurally related secreted glycoproteins. Wnt-1 and Wnt-3 were originally identified as oncogenes activated by the insertion of mouse mammary tumor virus in virus-induced mammary adenocarcinomas, although they are not expressed in the normal mammary gland. However, five other Wnt genes are differentially expressed during development of adult mammary tissue, suggesting that they may play distinct roles in various phases of mammary gland growth and development. Induction of transformation by Wnt-1 and Wnt-3 may be due to interference with these normal regulatory events; however, there is no direct evidence for this hypothesis. We have tested Wnt family members for the ability to induce transformation of cultured mammary cells. The results demonstrate that the Wnt gene family can be divided into three groups depending on their ability to induce morphological transformation and altered growth characteristics of the C57MG mammary epithelial cell line. Wnt-1, Wnt-3A, and Wnt-7A were highly transforming and induced colonies which formed and shed balls of cells. Wnt-2, Wnt-5B, and Wnt-7B also induced transformation but with a lower frequency and an apparent decrease in saturation density. In contrast, Wnt-6 and two other family members which are normally expressed in C57MG cells, Wnt-4 and Wnt-5A, failed to induce transformation. These data demonstrate that the Wnt genes have distinct effects on cell growth and should not be regarded as functionally equivalent.
机译：小鼠Wnt家族包括至少10个编码与结构相关的分泌糖蛋白的基因。 Wnt-1和Wnt-3最初被确定为通过在病毒诱导的乳腺腺癌中插入小鼠乳腺肿瘤病毒而激活的致癌基因，尽管它们在正常乳腺中未表达。然而，其他五个Wnt基因在成年乳腺组织的发育过程中差异表达，表明它们可能在乳腺生长和发育的各个阶段发挥不同的作用。 Wnt-1和Wnt-3诱导的转化可能是由于干扰了这些正常调控事件。但是，没有直接的证据支持这一假设。我们已经测试了Wnt家族成员诱导培养的乳腺细胞转化的能力。结果表明，Wnt基因家族可根据其诱导C57MG乳腺上皮细胞系形态转化和改变生长特性的能力分为三类。 Wnt-1，Wnt-3A和Wnt-7A高度转化并诱导形成和脱落细胞球的菌落。 Wnt-2，Wnt-5B和Wnt-7B也诱导了转化，但频率较低且饱和密度明显降低。相反，通常在C57MG细胞中表达的Wnt-6和其他两个家族成员Wnt-4和Wnt-5A无法诱导转化。这些数据表明，Wnt基因对细胞生长具有明显的影响，不应被视为功能等同。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号