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首页> 外文期刊>Molecular and Cellular Biology >Yeast mitochondria lacking the phosphate carrier/p32 are blocked in phosphate transport but can import preproteins after regeneration of a membrane potential.
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Yeast mitochondria lacking the phosphate carrier/p32 are blocked in phosphate transport but can import preproteins after regeneration of a membrane potential.

机译：缺乏磷酸盐载体/ p32的酵母线粒体在磷酸盐运输中受阻，但可以在膜电位再生后导入前蛋白。

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摘要

Two different functions have been proposed for the phosphate carrier protein/p32 of Saccharomyces cerevisiae mitochondria: transport of phosphate and requirement for import of precursor proteins into mitochondria. We characterized a yeast mutant lacking the gene for the phosphate carrier/p32 and found both a block in the import of phosphate and a strong reduction in the import of preproteins transported to the mitochondrial inner membrane and matrix. Binding of preproteins to the surface of mutant mitochondria and import of outer membrane proteins were not inhibited, indicating that the inhibition of protein import occurred after the recognition step at the outer membrane. The membrane potential across the inner membrane of the mutant mitochondria was strongly reduced. Restoration of the membrane potential restored preprotein import but did not affect the block of phosphate transport of the mutant mitochondria. We conclude that the inhibition of protein import into mitochondria lacking the phosphate carrier/p32 is indirectly caused by a reduction of the mitochondrial membrane potential (delta(gamma)), and we propose a model that the reduction of delta(psi) is due to the defective phosphate import, suggesting that phosphate transport is the primary function of the phosphate carrier/p32.
机译：对于酿酒酵母线粒体的磷酸盐载体蛋白/ p32，已经提出了两种不同的功能：磷酸盐的运输和将前体蛋白导入线粒体的需求。我们鉴定了一个缺少磷酸盐载体/ p32基因的酵母突变体，发现磷酸盐的导入受阻，而转运至线粒体内膜和基质的前蛋白的导入则大大减少。前蛋白与突变体线粒体表面的结合和外膜蛋白的导入均未受到抑制，这表明对蛋白导入的抑制发生在外膜识别步骤之后。跨突变线粒体内膜的膜电位大大降低。膜电位的恢复恢复了前蛋白的导入，但不影响突变型线粒体的磷酸盐转运。我们得出的结论是，缺乏磷酸盐载体/ p32的线粒体中蛋白质的导入抑制作用是由线粒体膜电位（delta（γ））的降低间接引起的，我们提出了一个模型，其delta（psi）的降低是由于有缺陷的磷酸盐进口，表明磷酸盐运输是磷酸盐载体/ p32的主要功能。

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