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首页> 外文期刊>Molecular and Cellular Biology >Non-Mendelian, heritable blocks to DNA rearrangement are induced by loading the somatic nucleus of Tetrahymena thermophila with germ line-limited DNA.
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Non-Mendelian, heritable blocks to DNA rearrangement are induced by loading the somatic nucleus of Tetrahymena thermophila with germ line-limited DNA.

机译：通过将嗜热四膜菌的体细胞核加载种系受限的DNA，可以诱导DNA重排的非孟德尔遗传性阻滞。

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摘要

Site-specific DNA deletion occurs at thousands of sites within the genome during macronuclear development of Tetrahymena thermophila. These deletion elements are usually not detected in macronuclear chromosomes. We have interfered with the normal deletion of two of these elements, the adjacent M and R elements, by loading vegetative macronuclei with these elements prior to sexual conjugation. Transformed cell lines containing the exogenous M or R element, carried on high-copy-number vectors containing genes encoding rRNA within parental (old) macronuclei, consistently failed to excise chromosomal copies of the M or R element during formation of new macronuclei. Little or no interference with the deletions of adjacent elements or of unlinked elements was observed. The micronucleus (germ line)-limited region of each element was sufficient to inhibit specific DNA deletion. This interference with DNA deletion usually is manifested as a cytoplasmic dominant trait: deletion elements present in the old macronucleus of one partner of a mating pair were sufficient to inhibit deletion occurring in the other partner. Remarkably, the failure to excise these elements became a non-Mendelian, inheritable trait in the next generation and did not require the high copy number of exogenously introduced elements. The introduction of exogenous deletion elements into parental macronuclei provides us with an epigenetic means to establish a heritable pattern of DNA rearrangement.
机译：在嗜热四膜虫的大核发育过程中，位点特异性DNA缺失发生在基因组内的数千个位点。通常在大核染色体中未检测到这些缺失元件。我们通过在性共轭之前将这些元素装载到植物大核上，干扰了其中两个元素（相邻的M和R元素）的正常删除。在亲本（旧）大核内包含编码rRNA基因的高拷贝数载体上携带的含有外源M或R元素的转化细胞系，在新的大核形成过程中始终无法切除M或R元素的染色体拷贝。观察到很少或没有干扰相邻元件或未连接元件的缺失。每个元素的微核（胚系）限制区足以抑制特定的DNA缺失。这种对DNA缺失的干扰通常表现为细胞质显性特征：存在于交配对的一个配偶的旧大核中的缺失元素足以抑制另一个配偶发生的缺失。值得注意的是，未能切除这些元素成为下一代的非孟德尔式可遗传特征，并且不需要大量复制的外源引入元素。将外源缺失元件引入亲本大细胞核为我们提供了表观遗传学的方法，以建立可遗传的DNA重排模式。

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