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Cell division induced by mechanical stimulation in starved Tetrahymena thermophila: cell cycle without synthesis of macronuclear DNA.

机译:饥饿的四膜虫嗜热菌中机械刺激诱导的细胞分裂:不合成大核DNA的细胞周期。

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摘要

Starved Tetrahymena thermophila cells underwent synchronous cell division 2 h after a mechanical stimulation. The macronucleus showed no obvious increase in DNA content before the cell division in the starvation medium, and the DNA content was decreased after the cell division. On the other hand, when the starved cells were given nutrient-supplied medium immediately after the mechanical stimulation, cell division was delayed for 3 h. This period was almost the same as that for G1 cells in the stationary culture to first division after transfer to fresh nutrient medium. These results suggest that the mechanical stimulation induces an early division of starved cells, skipping the macronuclear S-phase with the starved cells probably becoming trapped in G1. Starved cells that had finished division soon formed mating pairs with cells of the opposite type. These observations lead us to propose that cell division in starvation conditions may be necessary to reduce macronuclear DNA content prior to the mating of T. thermophila.
机译:机械刺激后2小时,饥饿的四膜虫嗜热菌细胞经历同步细胞分裂。饥饿培养基中细胞分裂前,大核DNA含量没有明显增加,细胞分裂后,DNA含量降低。另一方面,当饥饿的细胞在机械刺激后立即给予营养提供的培养基时,细胞分裂会延迟3小时。这个时期几乎与静止培养中转移到新鲜营养培养基后第一次分裂的G1细胞的时期相同。这些结果表明,机械刺激诱导了饥饿细胞的早期分裂,跳过了大核S期,饥饿细胞可能被困在G1中。完成分裂的饥饿细胞很快与相反类型的细胞形成配对。这些观察结果使我们提出,在饥饿条件下进行细胞分裂对于减少嗜热衣原体交配之前减少大核DNA含量可能是必要的。

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