...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Molecular and Cellular Biology >Interaction of TATA-Binding Protein with Upstream Activation Factor Is Required for Activated Transcription of Ribosomal DNA by RNA Polymerase I in Saccharomyces cerevisiae In Vivo
【24h】

Interaction of TATA-Binding Protein with Upstream Activation Factor Is Required for Activated Transcription of Ribosomal DNA by RNA Polymerase I in Saccharomyces cerevisiae In Vivo

机译：酿酒酵母体内RNA聚合酶I激活的核糖体DNA转录需要TATA结合蛋白与上游激活因子的相互作用。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Previous in vitro studies have shown that initiation of transcription of ribosomal DNA (rDNA) in the yeast Saccharomyces cerevisiae involves an interaction of upstream activation factor (UAF) with the upstream element of the promoter, forming a stable UAF-template complex; together with TATA-binding protein (TBP), UAF then recruits an essential factor, core factor (CF), to the promoter, forming a stable preinitiation complex. TBP interacts with both UAF and CF in vitro. In addition, a subunit of UAF, Rrn9p, interacts with TBP in vitro and in the two-hybrid system, suggesting the possible importance of this interaction for UAF function. Using the yeast two-hybrid system, we have identified three mutations inRRN9 that abolish the interaction of Rrn9p with TBP without affecting its interaction with Rrn10p, another subunit of UAF. Yeast cells containing any one of these individual mutations,L110S, L269P, or L274Q, did not show any growth defects. However, cells containing a combination ofL110S with one of the other two mutations showed a temperature-sensitive phenotype, and this phenotype was suppressed by fusing the mutant genes to SPT15, which encodes TBP. In addition, another mutation (F186S), which disrupts both Rrn9p-TBP and Rrn9p-Rrn10p interactions in the two-hybrid system, abolished UAF function in vivo, and this mutational defect was suppressed by fusion of the mutant gene to SPT15 combined with overexpression of Rrn10p. These experiments demonstrate that the interaction of UAF with TBP, which is presumably achieved by the interaction of Rrn9p with TBP, is indeed important for high-level transcription of rDNA by RNA polymerase I in vivo.
机译：之前的体外研究表明，酵母 Saccharomyces cerevisiae 中核糖体DNA（rDNA）的转录起始涉及上游激活因子（UAF）与启动子上游元素的相互作用，形成稳定的UAF。 -模板复杂;然后，UAF与TATA结合蛋白（TBP）一起向启动子募集必需因子核心因子（CF），形成稳定的预起始复合物。 TBP在体外与UAF和CF相互作用。此外，UAF的一个亚基Rrn9p在体外和双杂交系统中都与TBP相互作用，这表明这种相互作用对UAF功能的重要性。使用酵母双杂交系统，我们在 RRN9 中鉴定了三个突变，这些突变消除了Rrn9p与TBP的相互作用，而没有影响它与UAF的另一个亚基Rrn10p的相互作用。包含这些单个突变中的任何一个的酵母细胞 L110S ， L269P 或 L274Q ，均未显示任何生长缺陷。但是，包含 L110S 与其他两个突变之一的组合的细胞表现出对温度敏感的表型，并且通过将突变基因融合到 SPT15 来抑制该表型。编码TBP。此外，另一个突变（ F186S ）破坏了双杂交系统中的Rrn9p-TBP和Rrn9p-Rrn10p相互作用，废除了体内的UAF功能，并且这种突变缺陷被融合在一起抑制了。 SPT15 的突变基因与Rrn10p的过表达相结合。这些实验表明，UAF与TBP的相互作用（大概是通过Rrn9p与TBP的相互作用实现的）对于在体内RNA聚合酶I进行rDNA的高水平转录确实很重要。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号