• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Molecular and Cellular Biology >A Positive Regulator of Mitosis, Sok2, Functions as a Negative Regulator of Meiosis in Saccharomyces cerevisiae
【24h】

A Positive Regulator of Mitosis, Sok2, Functions as a Negative Regulator of Meiosis in Saccharomyces cerevisiae

机译：Sok2，有丝分裂的积极调节剂，在酿酒酵母中作为减数分裂的负调节剂。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The choice between meiosis and alternative developmental pathways in budding yeast depends on the expression and activity of transcriptional activator Ime1. The transcription of IME1is repressed in the presence of glucose, and a low basal level ofIME1 RNA is observed in vegetative cultures with acetate as the sole carbon source. IREu, a 32-bp element in the IME1promoter, exhibits upstream activation sequence activity depending on Msn2 and -4 and the presence of acetate. We show that in the presence of glucose IREu functions as a negative element and that Sok2 mediates this repression activity. We show that Sok2 associates with Msn2. Sok2 functions as a general repressor whose availability and activity depend on glucose. The activity of Sok2 as a repressor depends on phosphorylation of T598 by protein kinase A (PKA). Relief of repression of Sok2 depends on both the N-terminal domain of Sok2 and Ime1. In the absence of glucose and the presence of Ime1 Sok2 is converted to a weak activator. Overexpression of Sok2 or mild expression of Sok2 with its N-terminal domain deleted leads to a decrease in sporulation. Previously it was reported that overexpression of Sok2 suppresses the growth defect resulting from a temperature-sensitive PKA; thus Sok2 has a positive role in mitosis. We show that Candida albicansEfg1, a homolog of Sok2, complements sok2Δ in repressing IREu. Our results demonstrate that Sok2, a positive regulator of mitosis, and Efg1, a positive regulator of filamentation, function as negative regulators of meiosis. We suggest that cells use the same regulators with opposing effects to ensure that meiosis will be an alternative to mitosis.
机译：在发芽酵母中减数分裂和替代发育途径之间的选择取决于转录激活因子Ime1的表达和活性。在葡萄糖存在下， IME1 的转录受到抑制，在以醋酸盐为唯一碳源的营养培养物中，观察到低水平的 IME1 RNA基础水平。 IREu是 IME1 启动子中的32 bp元件，根据Msn2和-4以及乙酸盐的存在，显示出上游激活序列活性。我们显示，在葡萄糖存在下，IREu充当负元素，而Sok2介导这种抑制活性。我们显示Sok2与Msn2相关。 Sok2充当一般的阻遏物，其可用性和活性取决于葡萄糖。 Sok2作为阻遏物的活性取决于蛋白激酶A（PKA）对T598的磷酸化作用。 Sok2抑制的缓解取决于Sok2和Ime1的N末端域。在缺少葡萄糖和Ime1的情况下，Sok2会转化为弱激活剂。 Sok2的过表达或Sok2 N末端结构域缺失的轻度表达导致孢子形成减少。以前有报道说，Sok2的过表达抑制了对温度敏感的PKA引起的生长缺陷。因此，Sok2在有丝分裂中具有积极作用。我们显示，白色念珠菌 Efg1，Sok2的同源物，在抑制IREu时与 sok2 Δ互补。我们的结果表明，Sok2（有丝分裂的正向调节剂）和Efg1（丝状化的正向调节剂）充当减数分裂的负向调节剂。我们建议细胞使用具有相反作用的相同调节剂，以确保减数分裂将替代有丝分裂。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号