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首页> 外文期刊>Molecular and Cellular Biology >Screening for Modulators of Spermine Tolerance Identifies Sky1, the SR Protein Kinase of Saccharomyces cerevisiae, as a Regulator of Polyamine Transport and Ion Homeostasis
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Screening for Modulators of Spermine Tolerance Identifies Sky1, the SR Protein Kinase of Saccharomyces cerevisiae, as a Regulator of Polyamine Transport and Ion Homeostasis

机译：筛选精子耐受性调节剂可确定Sky1，酿酒酵母的SR蛋白激酶，作为多胺转运和离子稳态的调节剂。

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Although most cells are capable of transporting polyamines, the mechanism that regulates polyamine transport in eukaryotes is still largely unknown. Using a genetic screen for clones capable of restoring spermine sensitivity to spermine-tolerant mutants ofSaccharomyces cerevisiae, we have demonstrated that Sky1p, a recently identified SR protein kinase, is a key regulator of polyamine transport. Yeast cells deleted for SKY1 developed tolerance to toxic levels of spermine, while overexpression of Sky1p in wild-type cells increased their sensitivity to spermine. Expression of the wild-type Sky1p but not of a catalytically inactive mutant restored sensitivity to spermine. SKY1 disruption results in dramatically reduced uptake of spermine, spermidine, and putrescine. In addition to spermine tolerance, sky1Δ cells exhibit increased tolerance to lithium and sodium ions but somewhat increased sensitivity to osmotic shock. The observed halotolerance suggests potential regulatory interaction between the transport of polyamines and inorganic ions, as suggested in the case of the Ptk2p, a recently described regulator of polyamine transport. We demonstrate that these two kinases act in two different signaling pathways. While deletion or overexpression of SKY1 did not significantly affect Pma1p activity, the ability of overexpressed Sky1p, Ptk1p, and Ptk2p to increase sensitivity to LiCl depends on the integrity ofPPZ1 but not of ENA1.
机译：尽管大多数细胞都能够转运多胺，但在真核生物中调节多胺转运的机制仍然未知。使用能够恢复对精酵母耐受性的精胺突变体的精胺敏感性的克隆的遗传筛选，我们证明了最近鉴定出的SR蛋白激酶Sky1p是多胺转运的关键调节剂。删除了 SKY1 的酵母细胞对精胺的毒性水平具有耐受性，而野生型细胞中Sky1p的过表达增加了它们对精胺的敏感性。野生型Sky1p的表达，但没有催化失活的突变体的表达恢复了对精胺的敏感性。 SKY1 破坏会导致精胺，亚精胺和腐胺的摄取大大减少。除了对精胺的耐受性外， sky1 Δ细胞还表现出对锂和钠离子的耐受性增加，但对渗透压的敏感性有所提高。正如最近描述的多胺转运调节剂Ptk2p所表明的，观察到的耐盐性表明多胺和无机离子的转运之间存在潜在的调节相互作用。我们证明这两种激酶在两种不同的信号通路中起作用。尽管 SKY1 的缺失或过表达并没有显着影响Pma1p的活性，但是过表达的Sky1p，Ptk1p和Ptk2p增加对LiCl敏感性的能力取决于 PPZ1 的完整性。的 ENA1。

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