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首页> 外文期刊>Molecular and Cellular Biology >Regulation of the Premiddle and Middle Phases of Expression of the NDT80 Gene during Sporulation of Saccharomyces cerevisiae
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Regulation of the Premiddle and Middle Phases of Expression of the NDT80 Gene during Sporulation of Saccharomyces cerevisiae

机译：酿酒酵母孢子形成过程中NDT80基因表达的中上阶段的调节。

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The NDT80 gene of Saccharomyces cerevisiae, which encodes a global activator of transcription of middle sporulation-specific genes, is first expressed after the activation of early meiotic genes but prior to activation of middle sporulation-specific genes. Both upstream repression sequence 1 (URS1) and mid-sporulation element (MSE) sites are present in the promoter region of the NDT80 gene; these elements have been shown previously to contribute to the regulation of expression of early and middle sporulation-specific genes, respectively, by mediating repression in growing cells and activation at specific times during sporulation. In this study, we have shown that the overlapping windows of URS1- and MSE-mediated repression and activation are responsible for the distinctive premiddle expression pattern of the NDT80 gene. Our data suggest that a Sum1-associated repression complex bound at the NDT80 MSE sites prevents Ime1 tethered at the NDT80 URS1 sites from activating transcription of the NDT80 gene at the time that Ime1-dependent activation of early URS1-regulated meiotic genes is occurring. We propose that a decrease in the efficiency of Sum1-mediated repression as cells progress through the early events of the sporulation program allows the previously inactive Ime1 tethered at the URS1NDT80 sites to promote a low level of expression of the NDT80 gene. This initial phase of URS1-dependent NDT80 expression is followed by Ndt80-dependent upregulation of its own expression, which requires the MSENDT80 sites and occurs concomitantly with Ndt80-dependent activation of a set of middle MSE-regulated sporulation-specific genes. Mutation of IME2 prevents expression of NDT80 in sporulating cells. We show in this study that NDT80 is expressed and that middle genes are activated in cells of an Δime2/Δime2 Δsum1/Δsum1 strain in sporulation medium. This suggests that Ime2 activates expression of NDT80 by eliminating Sum1-mediated repression.
机译：酿酒酵母的 NDT80 基因编码中等孢子形成特异性基因的转录整体激活因子，在早期减数分裂基因激活后但在激活之前被首先表达。中等孢子特异性基因。 NDT80 基因的启动子区域中同时存在上游阻抑序列1（URS1）和中产子元件（MSE）。这些元素先前已显示出通过介导生长细胞中的阻遏和在孢子形成过程中特定时间的激活而分别对早期和中期孢子特异性基因表达的调节做出贡献。在这项研究中，我们已经表明，URS1和MSE介导的抑制和激活的重叠窗口是 NDT80 基因独特的中间表达模式的原因。我们的数据表明，与 NDT80 MSE位点结合的Sum1相关的阻遏复合物阻止了拴在 NDT80 URS1位点的Ime1激活 NDT80 发生早期URS1调节的减数分裂基因的Ime1依赖性激活时的>基因。我们建议，随着细胞在孢子形成程序的早期事件中进展，Sum1介导的阻遏效率降低，这使得先前在URS1 NDT80 站点上处于非活动状态的Ime1受到束缚。促进 NDT80 基因的低水平表达。依赖URS1的 NDT80 表达的初始阶段是其自身表达的Ndt80依赖性上调，这需要MSE NDT80 位点并发生与Ndt80依赖的一组中等MSE调控的孢子形成特异性基因的激活同时发生。 IME2 的突变可阻止 NDT80 在孢子形成细胞中表达。我们在这项研究中表明， NDT80 被表达并且中间基因在Δ ime2 / Δ ime2 Δ sum1的细胞中被激活/ Δ sum1 孢子形成培养基中的菌株。这表明Ime2通过消除Sum1介导的阻遏来激活 NDT80 的表达。

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