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首页> 外文期刊>Molecular and Cellular Biology >Actin gene expression in developing sea urchin embryos.
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Actin gene expression in developing sea urchin embryos.

机译：肌动蛋白基因在发育中的海胆胚胎中的表达。

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摘要

We show that the synthesis of actin is regulated developmentally during early sea urchin embryogenesis and that the level of synthesis of this protein parallels the steady-state amounts of the actin messenger ribonucleic acids (RNA). An in vitro translation and RNA blotting analysis of embryo RNA from several stages of early development indicated that during the first 8 h after fertilization there was a low and relatively constant level of actin messenger RNA in the embryo. Between 8 and 13 h of development, the amount of actin messenger RNA began to increase both in the cytoplasm and on polysomes, and by 18 h the amounts of actin message per embryo had risen between approximately 10- and 25-fold in the cytoplasm and between 15- and 40-fold on polysomes. Two size classes of actin messenger RNA (2.2 and 1.8 kilobases) were identified in unfertilized eggs and in all of the developmental stages examined. The amount of each actin message class increased over a similar time interval during early development. However, the amounts of these size classes in the cytoplasm relative to each other shifted between the earliest stages examined (2 to 5 h) and the hatching blastula stage (18 h), with the ratio of the 1.8-kilobase actin messenger RNA to the 2.2-kilobase actin messenger RNA increasing almost threefold during this period.
机译：我们显示肌动蛋白的合成在早期海胆胚胎发生过程中受到发育调控，并且该蛋白的合成水平与肌动蛋白信使核糖核酸（RNA）的稳态量平行。从早期发育的几个阶段进行的胚胎RNA的体外翻译和RNA印迹分析表明，受精后的最初8小时内，胚胎中的肌动蛋白信使RNA含量较低且相对恒定。在发育的8至13小时之间，肌动蛋白信使RNA的量在细胞质和多核糖体上都开始增加，到18小时，每个胚胎的肌动蛋白信使量在细胞质中增加了约10到25倍。在多核糖体上是15到40倍在未受精卵和所检查的所有发育阶段中，鉴定出两种大小的肌动蛋白信使RNA（2.2和1.8千碱基）。在早期发育期间，每个肌动蛋白信息类别的数量在相似的时间间隔内增加。然而，在细胞质中这些大小类别的数量相对于彼此在最早检查的阶段（2至5小时）和孵化的囊胚阶段（18小时）之间发生了变化，其中1.8千碱基肌动蛋白信使RNA与在此期间，2.2千碱基肌动蛋白信使RNA几乎增加了三倍。

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