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首页> 外文期刊>Molecular and Cellular Biology >Inhibition of Adenovirus DNA Synthesis In Vitro by Sera from Patients with Systemic Lupus Erythematosus
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Inhibition of Adenovirus DNA Synthesis In Vitro by Sera from Patients with Systemic Lupus Erythematosus

机译：系统性红斑狼疮患者血清对腺病毒DNA合成的抑制作用

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摘要

Sera containing antinuclear antibodies from patients with systemic lupus erythematosus (SLE) and related disorders were tested for their effect on the synthesis of adenovirus (Ad) DNA in an in vitro replication system. After being heated at 60°C for 1 h, some sera from patients with SLE inhibited Ad DNA synthesis by 60 to 100%. Antibodies to double-stranded DNA were present in 15 of the 16 inhibitory sera, and inhibitory activity copurified with anti-double-stranded DNA in the immunoglobulin G fraction. These SLE sera did not inhibit the DNA polymerases α, β, γ and had no antibody to the 72,000-dalton DNA-binding protein necessary for Ad DNA synthesis. The presence of antibodies to single-stranded DNA and a variety of saline-extractable antigens (Sm, Ha, nRNP, and rRNP) did not correlate with SLE serum inhibitory activity. Methods previously developed for studying the individual steps in Ad DNA replication were used to determine the site of inhibition by the SLE sera that contained antibody to double-stranded DNA. Concentrations of the SLE inhibitor that decreased the elongation of Ad DNA by greater than 85% had no effect on either the initiation of Ad DNA synthesis or the polymerization of the first 26 deoxyribonucleotides.
机译：在体外复制系统中，测试了含有系统性红斑狼疮（SLE）和相关疾病患者抗核抗体的血清对腺病毒（Ad）DNA合成的影响。在60°C加热1小时后，SLE患者的一些血清抑制了60％至100％的Ad DNA合成。 16种抑制性血清中有15种存在针对双链DNA的抗体，并且在免疫球蛋白G级分中与抗双链DNA共纯化了抑制活性。这些SLE血清不抑制DNA聚合酶α，β，γ，并且没有针对Ad DNA合成所必需的72,000道尔顿DNA结合蛋白的抗体。抗单链DNA抗体和各种盐提取抗原（Sm，Ha，nRNP和rRNP）的存在与SLE血清抑制活性无关。以前开发的用于研究Ad DNA复制中各个步骤的方法被用来确定SLE血清的抑制位点，该SLE血清中含有针对双链DNA的抗体。 SLE抑制剂的浓度使Ad DNA的延伸降低了85％以上，对Ad DNA合成的启动或前26个脱氧核糖核苷酸的聚合均没有影响。

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