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首页> 外文期刊>Molecular and Cellular Biology >Cycloheximide and heat shock induce new polypeptide synthesis in Neurospora crassa.
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Cycloheximide and heat shock induce new polypeptide synthesis in Neurospora crassa.

机译：环己二酰亚胺和热休克诱导了神经孢菌中新的多肽合成。

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摘要

Treatment of Neurospora crassa with 0.1 microgram of cycloheximide per ml, a concentration which inhibited protein synthesis by about 70%, resulted in the greatly enhanced synthesis of at least three polypeptide bands with estimated molecular weights of 88,000, 30,000, and 28,000. A temperature shift from 25 to 37 degrees C resulted in the appearance of a single new polypeptide band of 70,000 daltons, the same size as the major heat shock-induced proteins observed in species of Drosophila and Dictyostelium. Synthesis of the cycloheximide-stimulated polypeptide bands was on cytoplasmic ribosomes rather than on mitochondrial ribosomes, as incorporation of isotope into the polypeptide bands was inhibited by 1.0 microgram of cycloheximide per ml but not by 1 mg of chloramphenicol per ml. In a mutant with cycloheximide-resistant ribosomes, 0.1 microgram of cycloheximide per ml failed to alter the pattern of protein synthesis from that of the controls. It is suggested that the new synthesis of the polypeptide bands reflects specific mechanisms of adaptation to different kinds of environmental stress, including inhibition of protein synthesis and temperature increases.
机译：用每毫升0.1微克的环己酰亚胺（浓度可抑制约70％的蛋白质合成）处理crus Neurospora crassa，导致至少三个多肽条带的合成大大增强，估计分子量为88,000、30,000和28,000。从25摄氏度到37摄氏度的温度变化导致出现一条70,000道尔顿的新多肽条带，其大小与在果蝇和Dictyostelium物种中观察到的主要热激诱导蛋白相同。环己酰亚胺刺激的多肽条带的合成是在细胞质核糖体上，而不是在线粒体核糖体上，因为每毫升1.0微克环己酰亚胺可抑制同位素掺入多肽条带，而每毫升1mg氯霉素则不会。在具有抗环己酰亚胺抗性核糖体的突变体中，每毫升0.1微克环己酰亚胺未能改变对照蛋白质合成的模式。建议多肽条带的新合成反映了对不同种类环境胁迫的适应的特定机制，包括抑制蛋白质合成和温度升高。

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