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首页> 外文期刊>Molecular and Cellular Biology >Expression of a cell surface immobilization antigen during serotype transformation in Tetrahymena thermophila.
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Expression of a cell surface immobilization antigen during serotype transformation in Tetrahymena thermophila.

机译：嗜热四膜虫血清型转化过程中细胞表面固定抗原的表达。

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摘要

A temperature shift from 40 to 28 degrees C rapidly induced expression of a specific immobilization antigen at the cell surface in Tetrahymena thermophila. This transformation was inhibited by actinomycin D and cycloheximide but not by colchicine or cytochalasin B. The major surface antigen expressed at 28 degrees C in cells homozygous for the SerH3 allele was partially purified, and an antiserum against this preparation was raised in rabbits. Electrophoresis, immunoblot, and [35S]methionine incorporation studies are reported which support the conclusion that the H3 antigen is an acidic protein with an Mr of approximately 52,000 daltons. An induced synthesis of the H3 immobilization antigen was detected within 30 min after a shift from 40 to 28 degrees C. This protein appeared to be synthesized in the microsomal fraction and transferred without cleavage to the cell surface, where it was inserted first into nonciliated regions.
机译：从40摄氏度到28摄氏度的温度变化会迅速诱导嗜热四膜膜虫细胞表面特定固定抗原的表达。该转化受到放线菌素D和环己酰亚胺的抑制，但不受秋水仙碱或细胞松弛素B的抑制。在28摄氏度下针对SerH3等位基因纯合子的细胞中表达的主要表面抗原被部分纯化，并且在兔体内产生了针对该制剂的抗血清。据报道，电泳，免疫印迹和[35S]甲硫氨酸掺入研究均支持H3抗原是一种酸性蛋白质，Mr约为52,000道尔顿的结论。从40摄氏度转变为28摄氏度后30分钟内检测到诱导的H3固定抗原合成。这种蛋白质似乎是在微粒体级分中合成的，无需切割即可转移到细胞表面，首先将其插入非纤毛区域。 。

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