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首页> 外文期刊>Molecular and Cellular Biology >Rapid inhibition of processing and assembly of small nuclear ribonucleoproteins after infection with vesicular stomatitis virus.
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Rapid inhibition of processing and assembly of small nuclear ribonucleoproteins after infection with vesicular stomatitis virus.

机译：水泡性口腔炎病毒感染后，快速抑制小核糖核糖核蛋白的加工和装配。

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After infection of baby hamster kidney cells with vesicular stomatitis virus (VSV), processing and assembly of small nuclear ribonucleoproteins (snRNP) were rapidly inhibited. The U1 and U2 snRNAs accumulated as precursor species approximately 3 and 10 nucleotides longer, respectively, than the mature RNAs. Alteration in snRNP assembly was noted because the precursor snRNAs were not associated with the U-series RNA-core protein complex in infected cells. However, antibodies specific for the U2 RNA-binding protein, A', were able to precipitate pre-U2 RNAs from VSV-infected cells. These results indicated that precursors to U2 RNA were bound to A' and remained bound during virus infection. Analysis of the synthesis of proteins normally associated with U1 and U2 RNAs indicated that synthesis was unaffected at times when snRNP assembly with core proteins was blocked by the VSV. These findings suggested that the core proteins associate with one another in the absence of the snRNAs in VSV-infected cells. They further suggest a correlation between the inability of the core complex to bind the U-series snRNPs and the failure to process the 3' ends of U1 and U2 RNAs in VSV-infected cells. These effects of VSV on snRNP assembly may be related to the shutoff of host-cell macromolecular synthesis.
机译：在水泡性口腔炎病毒（VSV）感染小仓鼠肾细胞后，小核核糖核蛋白（snRNP）的加工和组装被迅速抑制。 U1和U2 snRNA作为前体物质积累的时间分别比成熟RNA长约3和10个核苷酸。注意到snRNP装配的变化，因为前体snRNA与感染细胞中的U系列RNA核心蛋白复合物不相关。但是，对U2 RNA结合蛋白A'特异的抗体能够从受VSV感染的细胞中沉淀U2前RNA。这些结果表明，U2 RNA的前体与A'结合并在病毒感染期间保持结合。对通常与U1和U2 RNA相关的蛋白质合成的分析表明，当核心蛋白的snRNP装配被VSV阻断时，合成不会受到影响。这些发现表明，在VSV感染的细胞中不存在snRNA的情况下，核心蛋白彼此缔合。他们进一步表明，核心复合物无法结合U系列snRNPs与在VSV感染的细胞中无法处理U1和U2 RNA的3'末端之间存在相关性。 VSV对snRNP组装的这些影响可能与宿主细胞大分子合成的关闭有关。

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