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首页> 外文期刊>Molecular and Cellular Biology >Gamma rays and bleomycin nick DNA and reverse the DNase I sensitivity of beta-globin gene chromatin in vivo.
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Gamma rays and bleomycin nick DNA and reverse the DNase I sensitivity of beta-globin gene chromatin in vivo.

机译：伽玛射线和博来霉素可切割DNA，并在体内逆转β-珠蛋白基因染色质对DNase I的敏感性。

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摘要

The active beta-globin genes in chicken erythrocytes, like all active genes, reside in large chromatin domains which are preferentially sensitive to digestion by DNase I. We have recently proposed that the special structure of chromatin in active domains is maintained by torsional stress in the DNA (Villeponteau et al., Cell 39:469-478, 1984). This hypothesis predicts that nicking of the DNA within any such chromosomal domain in vivo will relax the DNA and lead to loss of the special DNase I-sensitive state. Here we have tested this prediction by using gamma irradiation and bleomycin treatment to cleave DNA within intact chicken embryo erythrocytes. Both treatments cause reversal of DNase I sensitivity. Moreover, reversal occurs at approximately one nick per 150 kilobase pairs for both agents despite their entirely unrelated modes of cell penetration and DNA attack. These results suggest that the domain of DNase I sensitivity surrounding the beta-globin genes comprises 150 kilobase pairs of chromatin under torsional stress and that a single DNA nick in this region is sufficient to reverse the DNase I sensitivity throughout the entire domain.
机译：像所有活性基因一样，鸡红细胞中的活性β-珠蛋白基因也位于较大的染色质结构域中，该结构域对DNase I的消化优先敏感。我们最近提出，活性结构域中染色质的特殊结构通过扭转应力来维持。 DNA（Villeponteau等，Cell 39：469-478，1984）。该假设预测，体内任何此类染色体结构域内的DNA切口都会使DNA松弛，并导致特殊的DNase I敏感状态丧失。在这里，我们已经通过使用伽马射线照射和博来霉素处理来切割完整的鸡胚红细胞中的DNA来测试这一预测。两种处理均会导致DNase I敏感性逆转。而且，尽管两种试剂完全不相关的细胞渗透和DNA攻击方式，但它们在每150千碱基对中大约发生一个切口。这些结果表明，围绕β-球蛋白基因的DNase I敏感性结构域在扭转应力下包含150千碱基对的染色质，并且该区域中的单个DNA缺口足以在整个域中逆转DNase I敏感性。

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