Capturing nuclear sequence-specific DNA-binding proteins by using simian virus 40-derived minichromosomes.

U Nir; E Fodor; W J Rutter

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Capturing nuclear sequence-specific DNA-binding proteins by using simian virus 40-derived minichromosomes.

机译：通过使用猿猴病毒40衍生的微型染色体捕获核序列特异性DNA结合蛋白。

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We have used recombinant simian virus 40 (SV40) minichromosomes to retrieve sequence-specific DNA-binding proteins derived from the cell nucleus of COS-7 cells. We showed that the transcription factors AP-1 and Sp1 are stably bound to the SV40 DNA late in viral infection. Under similar conditions, minichromosomes carrying the rat insulin (rINS1) enhancer, which is under negative regulation in COS-7 cells, bound two proteins which mapped to distinct regions of the rINS1 enhancer. The SV40 P element competed for one of these proteins which bound to the region from -198 to -230. This factor may be related to AP-1. The other factor selectively bound a regulatory element in the region from -92 to -124 of the insulin enhancer. These proteins may play a role in regulating the rINS1 enhancer function.

机译：我们已经使用重组猿猴病毒40（SV40）微型染色体来检索从COS-7细胞的细胞核衍生的序列特异性DNA结合蛋白。我们显示转录因子AP-1和Sp1在病毒感染后期稳定地与SV40 DNA结合。在相似的条件下，携带大鼠胰岛素（rINS1）增强子的微型染色体在COS-7细胞中处于负调控状态，结合了两个蛋白质，它们映射到rINS1增强子的不同区域。 SV40 P元件竞争这些蛋白质中的一种，该蛋白质与-198至-230区域结合。此因素可能与AP-1有关。另一个因子选择性地结合胰岛素增强剂的-92至-124区域中的调节元件。这些蛋白质可能在调节rINS1增强子功能中发挥作用。

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《Molecular and Cellular Biology》 |1988年第2期|共6页
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U Nir; E Fodor; W J Rutter;
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中图分类细胞生物学;
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1. Sequence-specific interactions between a cellular DNA-binding protein and the simian virus 40 origin of DNA replication. [J] . W Traut, E Fanning Molecular and Cellular Biology . 1988,第2期

机译：细胞DNA结合蛋白与猿猴病毒40 DNA复制起点之间的序列特异性相互作用。
2. Binding of a sequence-specific single-stranded DNA-binding factor to the simian virus 40 core origin inverted repeat domain is cell cycle regulated. [J] . E P Carmichael, J M Roome, A F Wahl Molecular and Cellular Biology . 1993,第1期

机译：序列特异性单链DNA结合因子与猿猴病毒40核心来源的反向重复结构域的结合是细胞周期调节的。
3. Heterogeneous nuclear ribonucleoprotein D0B is a sequence-specific DNA-binding protein. [J] . Tolnay M, Vereshchagina LA, Tsokos GC The Biochemical Journal . 1999,第Pta2期

机译：异质核核糖核蛋白D0B是一种序列特异性DNA结合蛋白。
4. SEQUENCE-SPECIFIC CAPTURE OF PROTEIN-DNA COMPLEXES FOR MASS SPECTROMETRIC PROTEIN IDENTIFICATION [C] . Lloyd Smith, Siyuan Chen, Marla Chesnik, American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics . 2011

机译：质谱蛋白鉴定的蛋白质-DNA复合物的序列特异性捕获
5. THE EXPRESSION AND LOCALIZATION OF THE MAJOR DNA-BINDING PROTEIN OF HSV (VIROLOGY, CELL BIOLOGY, NUCLEAR ANTIGEN, HERPES SIMPLEX VIRUS) [D] . QUINLAN, MARGARET PATRICIA 1984

机译：HSV主要DNA结合蛋白（病毒学，细胞生物学，核抗原，单纯疱疹病毒）的表达和定位。
6. Capturing nuclear sequence-specific DNA-binding proteins by using simian virus 40-derived minichromosomes. [O] . U Nir, E Fodor, W J Rutter 1988

机译：通过使用猿猴病毒40衍生的微染色体捕获核序列特异性DNA结合蛋白。
7. Capturing nuclear sequence-specific DNA-binding proteins by using simian virus 40-derived minichromosomes. [O] . Nir, U, Fodor, E, Rutter, W J 1988

机译：通过使用猿猴病毒40衍生的微型染色体捕获核序列特异性DNA结合蛋白。

Capturing nuclear sequence-specific DNA-binding proteins by using simian virus 40-derived minichromosomes.

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