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首页> 外文期刊>Molecular and Cellular Biology >Unusual branch point selection in processing of human growth hormone pre-mRNA.
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Unusual branch point selection in processing of human growth hormone pre-mRNA.

机译：人类生长激素pre-mRNA加工中异常分支点的选择。

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摘要

Intron A of the human growth hormone gene does not contain an A residue within 56 nucleotides preceding the 3' splice site. The analysis of the excised intron lariat revealed a C residue 28 nucleotides upstream from the 3' splice site as the major branch acceptor nucleotide. Two additional minor branched nucleotides were identified as U residues at positions -22 and -36. An adenosine substitution at position -22 results in lariat formation solely to this nucleotide. Therefore, C and U residues can function efficiently as natural branch acceptors, but an A residue is preferred if available in the proper region. In addition, the data strongly reinforce the importance of the distance constraint for lariat formation. To explain selection of the branch acceptor nucleotide, potential base-pairing interactions of branch point sequences with the U2 RNA are discussed.
机译：人生长激素基因的内含子A在3'剪接位点之前的56个核苷酸内不包含A残基。切下的内含子套索的分析显示，在3'剪接位点上游28个核苷酸的C残基为主要的分支受体核苷酸。在位置-22和-36处的U残基被鉴定出另外两个较小的支链核苷酸。在-22位的腺苷取代仅导致该核苷酸的套索状形成。因此，C和U残基可以有效地用作天然支链受体，但是如果在适当区域可用，则A残基是优选的。另外，数据强烈地强调了距离约束对套索形成的重要性。为了解释分支受体核苷酸的选择，讨论了分支点序列与U2 RNA的潜在碱基配对相互作用。

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