Characterization of the promoter elements required for hepatic and intestinal transcription of the human apoB gene: definition of the DNA-binding site of a tissue-specific transcriptional factor.

D Kardassis; M Hadzopoulou-Cladaras; D P Ramji; R Cortese; V I Zannis; C Cladaras

首页> 外文期刊>Molecular and Cellular Biology >Characterization of the promoter elements required for hepatic and intestinal transcription of the human apoB gene: definition of the DNA-binding site of a tissue-specific transcriptional factor.

【24h】

Characterization of the promoter elements required for hepatic and intestinal transcription of the human apoB gene: definition of the DNA-binding site of a tissue-specific transcriptional factor.

机译：人apoB基因的肝和肠转录所需的启动子元件的特征：组织特异性转录因子的DNA结合位点的定义。

获取原文

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

The promoter elements important for intestinal and hepatic transcription of the human apoB gene have been localized downstream of nucleotide -150. Footprinting analysis using hepatic nuclear extracts identified four protected regions, -124 to -100, -97 to -93, -86 to -33, and +33 to +52. Gel electrophoretic mobility shift assays showed that multiple factors interact with the apoB sequence -86 to -33, while the region -88 to -61 binds a single nuclear factor. Methylation interference analysis and nucleotide substitution mutagenesis identified the binding site of the factor between residues -78 and -68. Binding competition experiments indicate that this factor recognizes the regulatory elements of other liver-specific genes.

机译：对于人apoB基因的肠道和肝脏转录重要的启动子元件已定位在核苷酸-150的下游。使用肝核提取物进行的足迹分析确定了四个保护区，-124至-100，-97至-93，-86至-33和+33至+52。凝胶电泳迁移率变动分析表明，多种因素与apoB序列-86至-33相互作用，而-88至-61区则与单个核因子结合。甲基化干扰分析和核苷酸取代诱变确定了残基-78和-68之间的因子结合位点。结合竞争实验表明该因子识别其他肝脏特异性基因的调控元件。

著录项

来源
《Molecular and Cellular Biology》 |1990年第6期|共7页
作者
D Kardassis; M Hadzopoulou-Cladaras; D P Ramji; R Cortese; V I Zannis; C Cladaras;
展开▼
作者单位

展开▼
收录信息
原文格式 PDF
正文语种
中图分类细胞生物学;
关键词

相似文献

外文文献
中文文献
专利

1. Correct usage of multiple transcription initiation sites and C/EBP-dependent transcription activation of the rat XDH/XO TATA-less promoter requires downstream elements located in the coding region of the gene. [J] . Clark MP, Chow CW, Rinaldo JE, Nucleic Acids Research . 1998,第7期

机译：正确使用大鼠XDH / XO TATA缺失型启动子的多个转录起始位点和C / EBP依赖性转录激活需要位于基因编码区的下游元件。
2. Correct usage of multiple transcription initiation sites and C/EBP-dependent transcription activation of the rat XDH/XO TATA-less promoter requires downstream elements located in the coding region of the gene [J] . Chi-Wing Chow, Jean E. Rinaldo, Melissa P. Clark, Nucleic acids research . 1998,第7期

机译：正确使用大鼠XDH / XO TATA缺失型启动子的多个转录起始位点和C / EBP依赖性转录激活需要位于基因编码区的下游元件
3. The binding site for the liver-specific transcription factor Tf-LF1 and the TATA box of the human transferrin gene promoter are the only elements necessary to direct liver-specific transcription in vitro [J] . Daniel Mendelzon, Fran?ois Boissier, Mario M. Zakin Nucleic acids research . 1990,第19期

机译：肝脏特异性转录因子Tf-LF1的结合位点和人转铁蛋白基因启动子的TATA框是在体外指导肝脏特异性转录的唯一要素
4. Identifying Transcription Factor Binding Sites in Promoters of Human Genes [C] . Wei Shi, Wanlei Zhou International Multi-Conference on Computing in the Global Information Technology . 2006

机译：鉴定人基因启动子中的转录因子结合位点
5. Identification and characterization of tissue-specific alternative promoter usage for Bcrp1/Abcg2 in mice for establishing a murine in vivo model of dietary transcriptional regulation of human small intestinal Bcrp/Abcg2 expression. [D] . Natarajan, Karthika. 2010

机译：Bcrp1 / Abcg2在小鼠中的组织特异性替代启动子用法的鉴定和表征，用于建立人体内小肠Bcrp / Abcg2表达的饮食转录调控的小鼠体内模型。
6. Characterization of the promoter elements required for hepatic and intestinal transcription of the human apoB gene: definition of the DNA-binding site of a tissue-specific transcriptional factor. [O] . D Kardassis, M Hadzopoulou-Cladaras, D P Ramji, 1990

机译：人apoB基因的肝和肠转录所需的启动子元件的特征：组织特异性转录因子的DNA结合位点的定义。
7. Characterization of the promoter elements required for hepatic and intestinal transcription of the human apoB gene: definition of the DNA-binding site of a tissue-specific transcriptional factor. [O] . Kardassis, D, Hadzopoulou-Cladaras, M, Ramji, D P, 1990

机译：人apoB基因的肝和肠转录所需的启动子元件的特征：组织特异性转录因子的DNA结合位点的定义。

Characterization of the promoter elements required for hepatic and intestinal transcription of the human apoB gene: definition of the DNA-binding site of a tissue-specific transcriptional factor.

摘要

著录项

相似文献

相关主题

期刊订阅